Identification, characterization, and expression analysis of adiponectin receptors in black carp Mylopharyngodon piceus in response to dietary carbohydrate
Identification, characterization, and expression analysis of adiponectin receptors in black carp Mylopharyngodon piceus in response to dietary carbohydrate
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青鱼青鱼脂联素受体对膳食碳水化合物反应的鉴定、表征和表达分析
DOI：
10.1007/s10695-018-0501-5
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发表时间：
2018-04
影响因子：
2.9
通讯作者：
Jinyun Ye
中科院分区：
文献类型：
--
作者：
Chenglong Wu;Jun’e Gao;Lian Chen;Xianping Shao;Jinyun Ye
本研究从青鱼mylophyngodon piceus幼鱼中分离到3个AdipoRs cdna （MpAdipoR1a、MpAdipoR1b和MpAdipoR2）。MpAdipoR1a、MpAdipoR1b和MpAdipoR2分别含有375、378和356个氨基酸。BLAST分析表明，mpadipor与斑马鱼、虹鳟鱼、人类等其他已知adipor具有很高的同源性。在mpadipor1和MpAdipoR2的氨基酸序列中分别存在7个跨膜区。MpAdipoR1s在黑鱼肝脏、血液、脑和眼睛中的相对表达量较高（p < 0.05）。MpAdipoR2在肝脏中表达较高（p < 0.05），其次是脂肪组织和血液。采用实时荧光定量PCR技术，分别在饲料碳水化合物（CHO）水平为10.65%、19.43%、28.84和37.91%的条件下饲养9周，检测肝脏、肌肉、脑、血液和脂肪组织中AdipoR mRNA的相对表达量。肝脏、肌肉、脑和血液中的MpAdipoR1s表达量均受到诱导，并在最优水平（19.43%或28.84%）达到最大值，在37.91%水平（19.43%、28.84%和37.91%）时有所下降，而肝脏中MpAdipoR1a表达量在19.43%、28.84%和37.91%水平间无显著差异。同样，与10.65%水平相比，最佳CHO水平下肝脏、肌肉、脑和血液中MpAdipoR2的表达量也达到了最高水平（19.43%或28.84%），但肝脏和肌肉中MpAdipoR2的表达量在饲料CHO水平为19.43%和37.91%组之间无显著差异（p < 0.05）。然而，与其他各组相比，过量膳食CHO显著下调脂肪组织中MpAdipoRs的表达水平（37.91%）（p < 0.05）。这些结果表明，mpadiprs是一种诱导蛋白，并有助于进一步了解其在调节黑鱼糖代谢稳态中的重要作用。
In the present study, three cDNAs of AdipoRs (MpAdipoR1a, MpAdipoR1b, and MpAdipoR2) were identified from juvenile black carp Mylopharyngodon piceus. There were 375, 378, and 356 amino acids in the MpAdipoR1a, MpAdipoR1b, and MpAdipoR2, respectively. BLAST analysis reveals that MpAdipoRs share high identities with other known AdipoRs from zebrafish, rainbow trout, human, etc. And there were all seven transmembrane regions in the amino acid sequences of MpAdipoR1s and MpAdipoR2, respectively. The relative expression levels of MpAdipoR1s were higher in the liver, blood, brain, and eyes in black carp (p < 0.05). Relatively higher expression of MpAdipoR2 was detected in the liver (p < 0.05) and then in the adipose tissues and blood by real-time PCR assays. The relative expression levels of AdipoR mRNA in the liver, muscle, brain, blood, and adipose tissues were detected by real-time PCR in black carp fed with four levels of dietary carbohydrate (CHO) (10.65, 19.43, 28.84, and 37.91%) for 9 weeks, respectively. The expression levels of MpAdipoR1s in the liver, muscle, brain, and blood were induced and reached to the maximum at optimal dietary CHO (19.43 or 28.84%) and then were decreased at 37.91% dietary CHO, although there were no significant differences on the expression levels of MpAdipoR1a in the liver between 19.43, 28.84, and 37.91% dietary CHO. Similarly, MpAdipoR2 were also induced to the maximal levels in the liver, muscle, brain, and blood at optimal dietary CHO (19.43 or 28.84%) compared with that at 10.65% dietary CHO, although no significant differences were observed on the expression levels of MpAdipoR2 in the liver and muscle between groups fed 19.43 and 37.91% dietary CHO (p > 0.05). However, the expression levels of MpAdipoRs in the adipose tissues were significantly downregulated at excessive dietary CHO (37.91%) compared with other groups (p < 0.05). These results indicated that MpAdipoRs were inducible proteins and might enhance the understanding of their vital roles in the regulation of glucose metabolic homeostasis in black carp.
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DOI：
10.1152/ajpregu.00745.2006
发表时间：
2007-07
期刊：
American journal of physiology. Regulatory, integrative and comparative physiology
影响因子：
--
作者：
R. Barazzoni;A. Bernardi;F. Biasia;A. Semolic;A. Bosutti;M. Mucci;F. Dore;M. Zanetti;G. Guarnieri
通讯作者：
R. Barazzoni;A. Bernardi;F. Biasia;A. Semolic;A. Bosutti;M. Mucci;F. Dore;M. Zanetti;G. Guarnieri
影响因子：
7.2
作者：
G. Szabadkai;M. Duchen
通讯作者：
G. Szabadkai;M. Duchen
影响因子：
4.7
作者：
Chenglong Wu;Jinyun Ye;Jun’e Gao;Lian Chen;Zhibin Lu
通讯作者：
Chenglong Wu;Jinyun Ye;Jun’e Gao;Lian Chen;Zhibin Lu
影响因子：
15.9
作者：
Furukawa, S.;Fujita, T.;Shimomura, I.
通讯作者：
Shimomura, I.
影响因子：
2.8
作者：
Chenglong Wu;Jinyun Ye;Jun’e Gao;Xia Yang;Yixiang Zhang
通讯作者：
Chenglong Wu;Jinyun Ye;Jun’e Gao;Xia Yang;Yixiang Zhang