Heat-stable molecule derived from Streptococcus cristatus induces APOBEC3 expression and inhibits HIV-1 replication.
Heat-stable molecule derived from Streptococcus cristatus induces APOBEC3 expression and inhibits HIV-1 replication.
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DOI：
10.1371/journal.pone.0106078
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Liu B
中科院分区：
文献类型：
--
作者：
Wang Z;Luo Y;Shao Q;Kinlock BL;Wang C;Hildreth JE;Xie H;Liu B
Although most human immunodeficiency virus type 1 (HIV-1) cases worldwide are transmitted through mucosal surfaces, transmission through the oral mucosal surface is a rare event. More than 700 bacterial species have been detected in the oral cavity. Despite great efforts to discover oral inhibitors of HIV, little information is available concerning the anti-HIV activity of oral bacterial components. Here we show that a molecule from an oral commensal bacterium, Streptococcus cristatus CC5A can induce expression of APOBEC3G (A3G) and APOBEC3F (A3F) and inhibit HIV-1 replication in THP-1 cells. We show by qRT-PCR that expression levels of A3G and A3F increase in a dose-dependent manner in the presence of a CC5A extract, as does A3G protein levels by Western blot assay. In addition, when the human monocytic cell line THP-1 was treated with CC5A extract, the replication of HIV-1 IIIB was significantly suppressed compared with IIIB replication in untreated THP-1 cells. Knock down of A3G expression in THP-1 cells compromised the ability of CC5A to inhibit HIV-1 IIIB infectivity. Furthermore, SupT1 cells infected with virus produced from CC5A extract-treated THP-1 cells replicated virus with a higher G to A hypermutation rate (a known consequence of A3G activity) than virus used from untreated THP-1 cells. This suggests that S. cristatus CC5A contains a molecule that induces A3G/F expression and thereby inhibits HIV replication. These findings might lead to the discovery of a novel anti-HIV/AIDS therapeutic.
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影响因子：
3.8
作者：
Liuzzi, G;Chirianni, A;Piazza, M
通讯作者：
Piazza, M
DOI：
10.1126/science.1232033
发表时间：
2013-02-15
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Mali P;Yang L;Esvelt KM;Aach J;Guell M;DiCarlo JE;Norville JE;Church GM
通讯作者：
Church GM
DOI：
10.1016/j.bbrc.2004.07.005
发表时间：
2004-08-27
影响因子：
3.1
作者：
Douaisi, M;Dussart, S;Decroly, E
通讯作者：
Decroly, E
影响因子：
56.9
作者：
FISHER, AG;ENSOLI, B;WONGSTAAL, F
通讯作者：
WONGSTAAL, F
影响因子：
4.8
作者：
Holmes, Rebecca K.;Koning, Fransje A.;Malim, Michael H.
通讯作者：
Malim, Michael H.