Initiation of meiosis and sporulation in Saccharomyces cerevisiae does not require a decrease in cyclic AMP
Initiation of meiosis and sporulation in Saccharomyces cerevisiae does not require a decrease in cyclic AMP
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酿酒酵母减数分裂和孢子形成的启动不需要环磷酸腺苷的减少
DOI：
10.1128/mcb.7.6.2141-2147.1987
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发表时间：
1987
影响因子：
5.3
通讯作者：
E. Freese
中科院分区：
文献类型：
--
作者：
Z. Olempska;E. Freese
酿酒酵母的减数分裂和孢子形成在鸟嘌呤营养缺陷型中通过鸟嘌呤剥夺而启动（E. Bautz Freese、Z. Olempska-Beer、A. Hartig 和 E. Freese, Dev. Biol. 102:438-451, 1984）。我们使用这种条件来检验一个假设（K. Matsumoto、I. Uno 和 T. Ishikawa，Cell 32：417-423，1983），即减数分裂的启动需要低水平的 cAMP。我们发现，鸟嘌呤剥夺后，细胞内 cAMP 浓度短暂下降不超过 20%，如果向培养基中添加 cAMP 磷酸二酯酶抑制剂 3-异丁基-1-甲基黄嘌呤 (IBMX)，则根本不会下降。在这些条件下，至少 76% 的细胞在没有 IBMX 的情况下形成孢子，而在存在 IBMX 的情况下几乎 100% 的细胞形成孢子。孢子细胞持续分泌cAMP并利用糖异生碳源。如果同时缺乏鸟嘌呤和碳，细胞就无法有效地形成孢子并形成四孢子子囊。在葡萄糖培养基中去除鸟嘌呤后，孢子形成仍然受到抑制，细胞内 cAMP 没有变化。我们的结论是，在鸟嘌呤饥饿的条件下，减数分裂和孢子形成的起始不需要cAMP缺乏，cAMP产生过量并排泄到培养基中，如果通过添加IBMX增加cAMP浓度，细胞会更好地产生孢子，细胞需要糖异生碳源来完成和有效的孢子形成，并且葡萄糖对孢子形成的抑制不是由cAMP介导的。
Meiosis and sporulation of Saccharomyces cerevisiae are initiated in a guanine auxotroph by guanine deprivation (E. Bautz Freese, Z. Olempska-Beer, A. Hartig, and E. Freese, Dev. Biol. 102:438-451, 1984). We used this condition to examine a hypothesis (K. Matsumoto, I. Uno, and T. Ishikawa, Cell 32:417-423, 1983) that initiation of meiosis requires a low level of cAMP. We found that, after guanine deprivation, the intracellular concentration of cAMP transiently decreased not more than 20% and not at all if the cAMP phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX) was added to the medium. Under these conditions, at least 76% of the cells sporulated in the absence of IBMX, and almost 100% sporulated in its presence. The sporulating cells continually excreted cAMP and utilized the gluconeogenic carbon source. The cells failed to sporulate efficiently and to form four-spored asci if simultaneously deprived of guanine and carbon. After guanine deprivation in glucose medium, sporulation remained suppressed and intracellular cAMP was unchanged. We conclude that, under conditions of guanine starvation, cAMP deficiency is not required for initiation of meiosis and sporulation, cAMP is produced in excess and excreted to the medium, the cells sporulate better if the cAMP concentration is increased by addition of IBMX, the cells require a gluconeogenic carbon source for complete and efficient sporulation, and suppression of sporulation by glucose is not mediated by cAMP.
酵母中ras基因的功能。
DOI：
10.1073/pnas.82.14.4740
发表时间：
1985
影响因子：
11.1
作者：
Fraenkel,DG
通讯作者：
Fraenkel,DG
酿酒酵母中的鸟嘌呤核苷酸敏感腺苷酸环化酶。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Casperson,GF;Walker,N;Brasier,AR;Bourne,HR
通讯作者：
Bourne,HR
酿酒酵母的 RAS2 是糖异生生长和对营养限制的适当反应所必需的。
DOI：
10.1073/pnas.82.11.3785
发表时间：
1985
影响因子：
11.1
作者：
Tatchell,K;Robinson,LC;Breitenbach,M
通讯作者：
Breitenbach,M