Role for chlamydial inclusion membrane proteins in inclusion membrane structure and biogenesis.
Role for chlamydial inclusion membrane proteins in inclusion membrane structure and biogenesis.
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DOI：
10.1371/journal.pone.0063426
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Hackstadt T
中科院分区：
文献类型：
--
作者：
Mital J;Miller NJ;Dorward DW;Dooley CA;Hackstadt T
衣原体包涵体膜通过插入 III 型分泌效应蛋白而被广泛修饰。这些包涵膜蛋白 (Incs) 暴露于胞质溶胶，具有长双叶疏水结构域的共同结构特征，但一级氨基酸序列相似性很少或没有。根据二级结构预测，沙眼衣原体中已鉴定出 50 多种推定的包涵膜蛋白。仅定义了有限数量的生物学功能，并且这些功能在衣原体物种之间不共享。在这里，我们在 HeLa 细胞中异位表达了几种沙眼衣原体 Incs，并发现它们诱导形态独特的膜性囊泡区室的形成。这些囊泡的形成至少需要双叶疏水结构域。没有观察到与这些囊泡相关的各种细胞器的标记。脂质探针由 Inc 诱导的囊泡掺入，尽管掺入的脂质取决于所表达的特定 Inc。 Inc对的共表达表明一些共定位于同一囊泡中，另一些部分重叠，还有一些根本不结合。总体而言，公司似乎具有诱导膜形成的内在能力，并且单个公司可以诱导具有独特性质的膜结构。
The chlamydial inclusion membrane is extensively modified by the insertion of type III secreted effector proteins. These inclusion membrane proteins (Incs) are exposed to the cytosol and share a common structural feature of a long, bi-lobed hydrophobic domain but little or no primary amino acid sequence similarity. Based upon secondary structural predictions, over 50 putative inclusion membrane proteins have been identified in Chlamydia trachomatis. Only a limited number of biological functions have been defined and these are not shared between chlamydial species. Here we have ectopically expressed several C. trachomatis Incs in HeLa cells and find that they induce the formation of morphologically distinct membranous vesicular compartments. Formation of these vesicles requires the bi-lobed hydrophobic domain as a minimum. No markers for various cellular organelles were observed in association with these vesicles. Lipid probes were incorporated by the Inc-induced vesicles although the lipids incorporated were dependent upon the specific Inc expressed. Co-expression of Inc pairs indicated that some colocalized in the same vesicle, others partially overlapped, and others did not associate at all. Overall, it appears that Incs may have an intrinsic ability to induce membrane formation and that individual Incs can induce membranous structures with unique properties.
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影响因子：
6.7
作者：
Derré I;Swiss R;Agaisse H
通讯作者：
Agaisse H
影响因子：
15.9
作者：
Hackstadt, T;Fischer, ER;Heinzen, RA
通讯作者：
Heinzen, RA
影响因子：
3.1
作者：
Cortes, Claudio;Rzomp, Mmberly A.;Wizel, Benjamin
通讯作者：
Wizel, Benjamin
影响因子：
4.8
作者：
Delevoye, C;Nilges, M;Subtil, A
通讯作者：
Subtil, A
DOI：
10.1073/pnas.92.11.4877
发表时间：
1995-05-23
影响因子：
11.1
作者：
HACKSTADT, T;SCIDMORE, MA;ROCKEY, DD
通讯作者：
ROCKEY, DD