The Toxoplasma gondii kinetochore is required for centrosome association with the centrocone (spindle pole).
The Toxoplasma gondii kinetochore is required for centrosome association with the centrocone (spindle pole).
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DOI：
10.1111/cmi.12185
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发表时间：
2014-01
影响因子：
3.4
通讯作者：
Gubbels MJ
中科院分区：
文献类型：
--
作者：
Farrell M;Gubbels MJ
着丝粒是一种装配在真核着丝粒上的多蛋白质结构，介导染色体与纺锤体微管的附着。在这里，我们鉴定了顶端复合体寄生虫弓形虫中的动粒蛋白Nuf2和Ndc80。定位显示，着丝点在整个细胞周期中保持簇状，并与中心锥的簇状着丝粒共定位，中心锥是一种含有嵌入核膜的纺锤体极的结构。微管的药理破坏导致部分着丝粒和着丝粒聚集的部分丧失，表明微管是着丝粒聚集所必需的，但不是严格要求的。TgNuf2条件基因敲除株的产生表明，TgNuf2对染色体分离是必不可少的，但对着丝粒聚集是必不可少的。着丝粒实际上仍然与着丝粒联系在一起，这表明它们与纺锤体极的相互作用不需要微管结合。对TgNuf2缺失最显著的观察是中心体行为正常，但它失去了与中心酮的联系。这表明，微管对于维持中心体和染色体之间的联系是必不可少的，这种相互作用对于将细胞核划分为两个子寄生虫至关重要。最后，用突变的TgNuf2结构进行的遗传互补实验突出了一个顶端复合体特异性基序，该基序可能在核定位中发挥作用。
The kinetochore is a multi-protein structure assembled on eukaryotic centromeres mediating chromosome attachment to spindle microtubules. Here we identified the kinetochore proteins Nuf2 and Ndc80 in the apicomplexan parasite Toxoplasma gondii. Localization revealed that kinetochores remain clustered throughout the cell cycle and co-localize with clustered centromeres at the centrocone, a structure containing the spindle pole embedded in the nuclear envelope. Pharmacological disruption of microtubules resulted in partial loss of some kinetochore and centromere clustering, indicating microtubules are necessary but not strictly required for kinetochore clustering. Generation of a TgNuf2 conditional knockdown strain revealed it is essential for chromosome segregation, but dispensable for centromere clustering. The centromeres actually remained associated with the centrocone suggesting microtubule binding is not required for their interaction with the spindle pole. The most striking observation upon TgNuf2 depletion was that the centrosome behaved normally, but that it lost its association with the centrocone. This suggests that microtubules are essential to maintain contact between the centrosome and chromosomes, and this interaction is critical for the partitioning of the nuclei into the two daughter parasites. Finally, genetic complementation experiments with mutated TgNuf2 constructs highlighted an apicomplexan specific motif with a putative role in nuclear localization.
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影响因子：
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DOI：
10.1016/b978-0-12-394309-5.00001-8
发表时间：
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影响因子：
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作者：
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通讯作者：
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