Liquid chromatography/mass spectrometry methods for measuring dipeptide abundance in non-small-cell lung cancer.
Liquid chromatography/mass spectrometry methods for measuring dipeptide abundance in non-small-cell lung cancer.
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DOI：
10.1002/rcm.6656
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发表时间：
2013-09-30
影响因子：
2
通讯作者：
Hoang, Chuong D.
中科院分区：
文献类型：
--
作者：
Wu, Manhong;Xu, Yue;Fitch, William L.;Zheng, Ming;Merritt, Robert E.;Shrager, Joseph B.;Zhang, Weiruo;Dill, David L.;Peltz, Gary;Hoang, Chuong D.
Metabolomic profiling is a promising methodology of identifying candidate biomarkers for disease detection and monitoring. Although lung cancer is among the leading causes of cancer-related mortality worldwide, the lung tumor metabolome has not been fully characterized. We utilized a targeted metabolomic approach to analyze discrete groups of related metabolites. We adopted a dansyl [5-(dimethylamino)-1-napthalene sulfonamide] derivatization with liquid chromatography/ mass spectrometry (LC/MS) to analyze changes of metabolites from paired tumor and normal lung tissues. Identification of dansylated dipeptides was confirmed with synthetic standards. A systematic analysis of retention times (RT) was required to reliably identify isobaric dipeptides. We validated our findings in a separate sample cohort. We produced a database of the LC retention time and MS/MS spectra of 361 dansyl dipeptides. Interpretation of the spectra is presented. Using this standard data, we identified a total of 279 dipeptides in lung tumor tissue. The abundance of 90 dipeptides was selectively increased in lung tumor tissue compared to normal tissue. In a second set of validation tissues, 12 dipeptides were selectively increased. A systematic evaluation of certain metabolite classes in lung tumors may identify promising disease-specific metabolites. Our database of all possible dipeptides will facilitate ongoing translational applications of metabolomic profiling as it relates to lung cancer.
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HMDB：人类代谢组数据库。
影响因子：
14.9
作者：
Wishart, David S;Tzur, Dan;Knox, Craig;Eisner, Roman;Guo, An Chi;Young, Nelson;Cheng, Dean;Jewell, Kevin;Arndt, David;Sawhney, Summit;Fung, Chris;Nikolai, Lisa;Lewis, Mike;Coutouly, Marie-Aude;Forsythe, Ian;Tang, Peter;Shrivastava, Savita;Jeroncic, Kevin;Stothard, Paul;Amegbey, Godwin;Block, David;Hau, David D;Wagner, James;Miniaci, Jessica;Clements, Melisa;Gebremedhin, Mulu;Guo, Natalie;Zhang, Ying;Duggan, Gavin E;Macinnis, Glen D;Weljie, Alim M;Dowlatabadi, Reza;Bamforth, Fiona;Clive, Derrick;Greiner, Russ;Li, Liang;Marrie, Tom;Sykes, Brian D;Vogel, Hans J;Querengesser, Lori
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Querengesser, Lori
影响因子：
5.3
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Hori, Suya;Nishiumi, Shin;Yoshida, Masaru
通讯作者：
Yoshida, Masaru
影响因子：
2.9
作者：
Tinoco, Arthur D.;Saghatelian, Alan
通讯作者：
Saghatelian, Alan
影响因子：
3.6
作者：
Fan, Teresa W. -M.;Lane, Andrew N.;Higashi, Richard M.;Bousamra, Michael, II;Kloecker, Goetz;Miller, Donald M.
通讯作者：
Miller, Donald M.
DOI：
10.1093/jee/39.2.269
发表时间：
1945-01-01
期刊：
BIOMETRICS BULLETIN
影响因子：
--
作者：
WILCOXON, F
通讯作者：
WILCOXON, F