Meiosis-specific cohesin mediates homolog recognition in mouse spermatocytes.
Meiosis-specific cohesin mediates homolog recognition in mouse spermatocytes.
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DOI：
10.1101/gad.237313.113
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发表时间：
2014-03-15
影响因子：
10.5
通讯作者：
Watanabe Y
中科院分区：
文献类型：
--
作者：
Ishiguro K;Kim J;Shibuya H;Hernández-Hernández A;Suzuki A;Fukagawa T;Shioi G;Kiyonari H;Li XC;Schimenti J;Höög C;Watanabe Y
同源染色体在减数分裂过程中如何相互识别仍然是染色体生物学的一个基本问题。石黑浩等人。现在表明，精母细胞中的同源识别始于早期减数分裂前期。有趣的是，早在 DNA 双链断裂形成之前，减数分裂特异性粘连蛋白就在同源物识别中发挥着重要作用。研究结果表明，同源识别可能主要是通过在减数分裂特异性粘连蛋白定义的染色体结构中寻找同源性来实现的，而不是通过 DNA 序列本身来实现。在减数分裂期间，同源染色体（同源物）配对受到多层调控的促进，包括动态染色体运动和减数分裂重组。然而，同源物相互识别的方式仍然是染色体生物学的一个基本问题。在这里，我们表明同源识别或关联在轴组装和双链断裂（DSB）形成之前进入减数分裂前期时启动。这种同源关联仅在轴形成期间或之后发展成紧密配对。有趣的是，Sun1 敲除精母细胞中保留了识别同源物的能力，其中端粒定向的染色体运动被废除，甚至在 Spo11 敲除精母细胞中也是如此，其中缺乏 DSB 依赖性 DNA 同源性搜索。减数分裂特异性粘连蛋白 RAD21L 的破坏会阻止同源物的初始关联以及精母细胞中的后续配对。这些发现表明了一个有趣的可能性，即同源识别主要是通过在减数分裂特异性粘连蛋白定义的染色体结构中寻找同源性来实现的，而不是在 DNA 序列本身中。
How homologous chromosomes recognize each other during meiosis remains a fundamental question in chromosome biology. Ishiguro et al. now show that homolog recognition in spermatocytes starts in early meiotic prophase. Intriguingly, meiosis-specific cohesin plays an essential role in homolog recognition far prior to DNA double-strand break formation. The findings suggest the possibility that homolog recognition is achieved primarily by searching for homology in the chromosome architecture as defined by meiosis-specific cohesin rather than the DNA sequence itself. During meiosis, homologous chromosome (homolog) pairing is promoted by several layers of regulation that include dynamic chromosome movement and meiotic recombination. However, the way in which homologs recognize each other remains a fundamental issue in chromosome biology. Here, we show that homolog recognition or association initiates upon entry into meiotic prophase before axis assembly and double-strand break (DSB) formation. This homolog association develops into tight pairing only during or after axis formation. Intriguingly, the ability to recognize homologs is retained in Sun1 knockout spermatocytes, in which telomere-directed chromosome movement is abolished, and this is the case even in Spo11 knockout spermatocytes, in which DSB-dependent DNA homology search is absent. Disruption of meiosis-specific cohesin RAD21L precludes the initial association of homologs as well as the subsequent pairing in spermatocytes. These findings suggest the intriguing possibility that homolog recognition is achieved primarily by searching for homology in the chromosome architecture as defined by meiosis-specific cohesin rather than in the DNA sequence itself.
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影响因子：
11.4
作者：
Herran, Yurema;Gutierrez-Caballero, Cristina;Pendas, Alberto M.
通讯作者：
Pendas, Alberto M.
DOI：
10.1073/pnas.0500172102
发表时间：
2005-03-22
影响因子：
11.1
作者：
Anderson, LK;Royer, SM;Hawley, RS
通讯作者：
Hawley, RS
影响因子：
1.5
作者：
Bannister, LA;Reinholdt, LG;Schimenti, JC
通讯作者：
Schimenti, JC
DOI：
10.1073/pnas.0606165104
发表时间：
2007-05-22
影响因子：
11.1
作者：
Conrad, Michael N.;Lee, Chih-Ying;Dresser, Michael E.
通讯作者：
Dresser, Michael E.
影响因子：
11.8
作者：
Ding, DQ;Yamamoto, A;Hiraoka, Y
通讯作者：
Hiraoka, Y