Two cyanobacterial response regulators with diguanylate cyclase activity, Rre2 and Rre8, participate in biofilm formation
Two cyanobacterial response regulators with diguanylate cyclase activity, Rre2 and Rre8, participate in biofilm formation
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两个具有二鸟苷酸环化酶活性的蓝藻反应调节因子 Rre2 和 Rre8 参与生物膜形成
DOI：
10.1111/mmi.15057
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发表时间：
2023
影响因子：
3.6
通讯作者：
Uozumi Nobuyuki
中科院分区：
文献类型：
--
作者：
Kobayashi Ayumu;Nakamura Masamune;Tsujii Masaru;Makino Kohei;Nagayama Tatsuya;Nakamura Kensuke;Nanatani Kei;Kota Kera;Furuuchi Yuki;Kayamori Shunsuke;Furuta Tadaomi;Suzuki Iwane;Hayakawa Yoshihiro;Tanudjaja Ellen;Ishimaru Yasuhiro;Uozumi Nobuyuki
光养细菌面临昼夜变化的环境条件，如光和渗透压，影响其碳代谢和产生有机化合物的能力。蓝藻的模式，聚囊藻。PCC 6803在高盐胁迫等极端条件下形成生物膜，但其感知环境变化导致生物膜形成的分子机制尚不清楚。在这里，我们研究了两个双组分调控系统（TCSs），它们含有二胍酸环化酶（DGCs），其产生第二信使c - di - GMP，作为聚胞菌生物膜诱导信号通路的潜在组分。单突变体的分析提供了应答调节因子Rre2和Rre8参与生物膜形成的证据。细菌双杂交实验表明，Rre2和Rre8分别与特定的组氨酸激酶Hik12和Hik14形成TCS。体外实验表明，无论Rre2的去磷酸化状态如何，Rre2都具有DGC活性，而Rre8则需要磷酸化才能具有DGC活性。Hik14‐Rre8可能是一种响应环境变化的诱导传感系统。协同胞囊突变体的生物膜实验表明，hik12‐rre2和hik14‐rre8对高盐度诱导的生物膜形成有反应。hik12‐rre2和hik14‐rre8的失活对光合作用的光反应性能没有影响。这些数据表明，Hik12‐Rre2和Hik14‐Rre8通过Rre2和Rre8的DGC活性调节c‐di‐GMP的产生，从而参与协同胞囊生物膜的形成。
Phototrophic bacteria face diurnal variations of environmental conditions such as light and osmolarity that affect their carbon metabolism and ability to generate organic compounds. The model cyanobacterium,Synechocystissp. PCC 6803 forms a biofilm when it encounters extreme conditions like high salt stress, but the molecular mechanisms involved in perception of environmental changes that lead to biofilm formation are unknown. Here, we studied two two‐component regulatory systems (TCSs) that contain diguanylate cyclases (DGCs), which produce the second messenger c‐di‐GMP, as potential components of the biofilm‐inducing signaling pathway inSynechocystis. Analysis of single mutants provided evidence for involvement of the response regulators, Rre2 and Rre8 in biofilm formation. A bacterial two‐hybrid assay showed that Rre2 and Rre8 each formed a TCS with a specific histidine kinase, Hik12 and Hik14, respectively. The in vitro assay showed that Rre2 had DGC activity regardless of its de/phosphorylation status, whereas Rre8 required phosphorylation for DGC activity. Hik14‐Rre8 likely functioned as an inducible sensing system in response to environmental change. Biofilm assays withSynechocystismutants suggested that pairs ofhik12‐rre2andhik14‐rre8responded to high salinity‐induced biofilm formation. Inactivation ofhik12‐rre2andhik14‐rre8did not affect the performance of the light reactions of photosynthesis. These data suggest that Hik12‐Rre2 and Hik14‐Rre8 participate in biofilm formation inSynechocystisby regulating c‐di‐GMP production via the DGC activity of Rre2 and Rre8.
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影响因子：
3.5
作者：
Motohashi K
通讯作者：
Motohashi K
影响因子：
3.6
作者：
M. van de Mortel;L. Halverson
通讯作者：
M. van de Mortel;L. Halverson
Tlr0485 是蓝细菌热聚球藻中的 cAMP 激活的 c-di-GMP 磷酸二酯酶。
DOI：
10.2323/jgam.2020.01.007
发表时间：
2020
期刊：
The Journal of general and applied microbiology
影响因子：
--
作者：
Gen Enomoto;A. Kamiya;Yukiko Okuda;R. Narikawa;M. Ikeuchi
通讯作者：
M. Ikeuchi
影响因子：
7.7
作者：
Bridges AA;Bassler BL
通讯作者：
Bassler BL
一种在实验室条件下产生集胞藻 PCC6803 生物膜的简单方法，用于电子显微镜和功能研究
影响因子：
3.7
作者：
I. Mallick;Prithwiraj Kirtania;M. Szabó;F. Bashir;I. Domonkos;P. Kós;I. Vass
通讯作者：
I. Vass