Structural Variants and Selective Sweep Foci Contribute to Insecticide Resistance in the Drosophila Genetic Reference Panel.
Structural Variants and Selective Sweep Foci Contribute to Insecticide Resistance in the Drosophila Genetic Reference Panel.
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结构变体和选择性扫描灶有助于果蝇遗传参考面板的杀虫剂耐药性。
DOI：
10.1534/g3.118.200619
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发表时间：
2018-11-06
期刊：
影响因子：
--
通讯作者：
Robin C
中科院分区：
文献类型：
--
作者：
Battlay P;Leblanc PB;Green L;Garud NR;Schmidt JM;Fournier-Level A;Robin C
黑腹果蝇种群内的核苷酸多态性模式表明，杀虫剂一直是驱动最近最强的正选择的选择剂。然而，需要明确地将选择性扫描与特定的杀虫剂表型联系起来，这可以合理地解释在这些非靶标昆虫中观察到的剧烈选择性反应。在这里，我们用两种常见的杀虫剂筛选果蝇遗传参考面板;马拉硫磷（一种有机磷酸酯）和氯菊酯（一种拟除虫菊酯）。全基因组关联研究将马拉硫磷的存活率映射到D。黑腹果蝇基因组; Ace和Cyp 6 g1。马拉硫磷存活率也与Cyp 12 d1、Jheh 1和Jheh 2转录丰度水平高的品系相关。氯菊酯表型映射到果蝇基因组中最大的P450基因簇，然而与杀虫剂使用驱动的选择性扫描相反，衍生的等位基因似乎与易感性相关。这些结果强调了以前的研究结果，突出了杀虫剂表型的结构变异的重要性：Cyp 6 g1表现出拷贝数变异和转座因子插入，Cyp 12 d1串联重复，Jheh基因座与Bari 1转座因子插入，Cyp 6a 17缺失与易感性相关。
Patterns of nucleotide polymorphism within populations of Drosophila melanogaster suggest that insecticides have been the selective agents driving the strongest recent bouts of positive selection. However, there is a need to explicitly link selective sweeps to the particular insecticide phenotypes that could plausibly account for the drastic selective responses that are observed in these non-target insects. Here, we screen the Drosophila Genetic Reference Panel with two common insecticides; malathion (an organophosphate) and permethrin (a pyrethroid). Genome-wide association studies map survival on malathion to two of the largest sweeps in the D. melanogaster genome; Ace and Cyp6g1. Malathion survivorship also correlates with lines which have high levels of Cyp12d1, Jheh1 and Jheh2 transcript abundance. Permethrin phenotypes map to the largest cluster of P450 genes in the Drosophila genome, however in contrast to a selective sweep driven by insecticide use, the derived allele seems to be associated with susceptibility. These results underscore previous findings that highlight the importance of structural variation to insecticide phenotypes: Cyp6g1 exhibits copy number variation and transposable element insertions, Cyp12d1 is tandemly duplicated, the Jheh loci are associated with a Bari1 transposable element insertion, and a Cyp6a17 deletion is associated with susceptibility.
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影响因子：
3.3
作者：
Garud, Nandita R.;Petrov, Dmitri A.
通讯作者：
Petrov, Dmitri A.
影响因子：
4.6
作者：
Denecke S;Fusetto R;Martelli F;Giang A;Battlay P;Fournier-Level A;O' Hair RA;Batterham P
通讯作者：
Batterham P
影响因子：
30.8
作者：
Chakraborty M;VanKuren NW;Zhao R;Zhang X;Kalsow S;Emerson JJ
通讯作者：
Emerson JJ
DOI：
10.1534/g3.116.031054
发表时间：
2016-08-09
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Battlay P;Schmidt JM;Fournier-Level A;Robin C
通讯作者：
Robin C
影响因子：
7
作者：
Faucon F;Dusfour I;Gaude T;Navratil V;Boyer F;Chandre F;Sirisopa P;Thanispong K;Juntarajumnong W;Poupardin R;Chareonviriyaphap T;Girod R;Corbel V;Reynaud S;David JP
通讯作者：
David JP