Progression of hepatic neoplasia in medaka (Oryzias latipes) exposed to diethylnitrosamine.
Progression of hepatic neoplasia in medaka (Oryzias latipes) exposed to diethylnitrosamine.
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暴露于二乙基亚硝胺的青鳉（Oryzias latipes）肝脏肿瘤的进展。
DOI：
10.1093/carcin/20.6.933
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发表时间：
1999
期刊：
影响因子：
4.7
通讯作者：
Hinton,DE
中科院分区：
文献类型：
--
作者：
Okihiro,MS;Hinton,DE
Progression of hepatic neoplasia was assessed in medaka (Oryzias latipes) following aqueous exposure to diethylnitrosamine (DEN). Larvae (2 weeks old) were exposed to 350 or 500 p.p.m. DEN for 48 h, while adults (3–6 months old) were exposed to 50 p.p.m. DEN for 5 weeks. Fish were maintained as long as possible to determine malignant potential of resultant neoplasms. A total of 423 medaka with 106 hepatic neoplasms were examined. There were marked differences in tumor prevalence between exposure groups including: (i) higher prevalence of hepatocellular carcinomas in medaka exposed as adults (100% of hepatocellular tumors in adult-exposed medaka were malignant, while only 51.5% of larval hepatocellular tumors were malignant); (ii) higher prevalence of biliary tumors in medaka exposed as larvae (46.4% of all tumors in larval-exposed medaka were biliary versus 8.1% in adult-exposed fish); (iii) higher prevalence of mixed hepato-biliary carcinomas in adult-exposed medaka (24.3%) compared with those exposed as larvae (3%). In addition, a unique hepatocellular lesion termed `nodular proliferation' was only observed in adult-exposed medaka. The lesion was characterized by small size (50–300 μm), complete loss of normal tubular architecture and variable megalocytosis. Nodular proliferation was distinct from preneoplastic foci of cellular alteration and may represent microcarcinomas. There was a step-wise increase in mean diameter with age (days post-exposure) from nodular proliferation (174 μm, 17 days) to hepatocellular carcinoma (1856 μm, 62 days) and mixed carcinomas (3209 μm, 93 days) in adult-exposed medaka. Metastasis was observed with 19 neoplasms and tumors with the highest metastatic potential were hepatocellular and mixed carcinomas. The most common form of metastasis was trans-coelomic, followed by direct invasion and distant metastasis, presumably via the vascular route. Differences in tumor prevalence between exposure groups were believed to be the result of length of DEN exposure rather than age of fish at the time of exposure. In larval medaka with brief (48 h) DEN exposure, neoplasms are thought to be the result of dedifferentiation of hepatic cells, with slow progression of foci of cellular alteration to benign and then malignant tumors. In contrast, with adult medaka and prolonged (5 week) DEN exposure, neoplasms are believed to result from initiation of committed stem cells and formation of microcarcinomas (`nodular proliferation'), before progressing to larger hepatocellular and then mixed carcinomas.
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二乙基亚硝胺处理期间和之后的温度对Oryzias latipes 鱼肝脏肿瘤发生的影响。
影响因子：
8.4
作者：
Y. Kyono
通讯作者：
Y. Kyono
水氯化 — 化学、环境影响和健康影响：Jolley, R. L.、Bull, R. J.、Katz, S.、Roberts, M. H. 和 Jacobs, V. A. 第 5 卷，1575 页。切尔西，密歇根州：Lewis Publishers, Inc. (1985)
影响因子：
2.8
作者：
J. Meier
通讯作者：
J. Meier
二乙基亚硝胺诱导的稻鳀肝脏肿瘤发生过程中肝细胞标记指数和DNA含量的变化
DOI：
10.1093/jnci/63.1.71
发表时间：
1979
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
Y. Kyono;A. Shima;N. Egami
通讯作者：
N. Egami
肝再生和肝癌发生中的癌基因表达。
DOI：
10.1002/hep.1840030621
发表时间：
1983
期刊：
Hepatology (Baltimore, Md.)
影响因子：
--
作者：
Fausto,N;Shank,PR
通讯作者：
Shank,PR
DOI：
--
发表时间：
1981
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
M. Lipsky;D. Hinton;J. Klaunig;P. Goldblatt;B. Trump
通讯作者：
B. Trump