Quantitative analysis of the immune response upon Salmonella typhimurium infection along the porcine intestinal gut.
Quantitative analysis of the immune response upon Salmonella typhimurium infection along the porcine intestinal gut.
复制标题
DOI：
10.1051/vetres/2009072
复制
发表时间：
2010-03
影响因子：
4.4
通讯作者：
Garrido JJ
中科院分区：
文献类型：
--
作者：
Collado-Romero M;Arce C;Ramírez-Boo M;Carvajal A;Garrido JJ
鼠伤寒沙门氏菌引起肠道疾病，危害食品安全。在猪中，肠对S.鼠伤寒的传统特征在于体外模型不能反映肠粘膜的实际免疫能力。在这项工作中，我们进行了口头S。鼠伤寒杆菌感染研究，以了解猪肠的三个不同部分（空肠、回肠和结肠）的体外反应。为此，在包括接种后1、2和6天的时间过程期间收集来自1月龄感染仔猪的样品，以通过使用定量实时PCR测定定量编码28种先天免疫系统分子的基因的mRNA表达来评价肠应答。此外，还使用来自未感染对照动物的样品来确定肠切片之间稳态基因表达的差异。定量分子组包括细胞因子、趋化因子、模式识别受体、细胞内信号分子、转录因子和抗菌分子的分类。基因表达的变化发生在肠道的三个不同部分，并在S。鼠伤寒感染此外，在编码炎症介质的基因的表达模式中观察到的高度变化可能表明每个肠道部分对感染的反应不同。因此，与空肠和结肠中的发现相反，在回肠中观察到一些促炎细胞因子转录物的下调和缺乏诱导。然而，在回肠和空肠中检测的所有趋化因子均上调，而在结肠中仅IL-8和MIP-1α mRNA过表达。总之，我们的研究结果揭示了猪肠道基因表达谱沿着的区域差异以及对S.鼠伤寒感染综上所述，这些数据应提供一个基础，为猪肠道细菌感染的反应的完整理解。
Salmonella enterica serovar Typhimurium causes enteric disease and compromises food safety. In pigs, the molecular response of the intestine to S. typhimurium has been traditionally characterized by in vitro models that do not reflect the actual immunological competence of the intestinal mucosa. In this work, we performed an oral S. typhimurium infection study to obtain insight into the in vitro response in three different sections (jejunum, ileum and colon) of the porcine intestine. For this, samples from one-month-old infected piglets were collected during a time course comprising 1, 2, and 6 days post inoculation to evaluate the intestinal response by quantifying the mRNA expression of gene coding for 28 innate immune system molecules using quantitative real-time PCR assays. In addition, samples from non-infected control animals were also employed to establish differences in the steady state gene expression between intestinal sections. The panel of quantified molecules included an assortment of cytokines, chemokines, pattern-recognition receptors, intracellular signaling molecules, transcription factors and antimicrobial molecules. Changes in gene expression occurred in the three different parts of the intestine and during the course of the S. typhimurium infection. Moreover, the high variation observed in expression patterns of genes coding for inflammatory mediators could indicate that each intestinal section responds differently to the infection. Thus, on the contrary to findings in the jejunum and colon, a down-regulation and lack of induction of some proinflammatory cytokine transcripts was observed in the ileum. Nevertheless, all chemoattractant cytokines assayed were up-regulated in the ileum and jejunum whereas only interleukin-8 and MIP-1α mRNA were over expressed in the colon. In conclusion, our results reveal regional differences in gene expression profiles along the porcine intestinal gut as well as regional differences in the inflammatory response to S. typhimurium infection. Taken together, these data should provide a basis for a complete understanding of the porcine intestinal response to bacterial infection.
登录
查看更多内容
DOI：
10.1098/rstb.2000.0603
发表时间：
2000-05-29
期刊：
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
Finlay, BB;Brumell, JH
通讯作者：
Brumell, JH
影响因子：
5.8
作者：
Jepson, MA;Clark, MA
通讯作者：
Clark, MA
影响因子：
1.8
作者：
Skjolaas, K. A.;Burkey, T. E.;Minton, J. E.
通讯作者：
Minton, J. E.
影响因子：
5.5
作者：
Keshav, Satish
通讯作者：
Keshav, Satish
影响因子：
2.5
作者：
Eckmann, L
通讯作者：
Eckmann, L