Genome-wide identification of the MADS-box transcription factor family in pear (Pyrus bretschneideri) reveals evolution and functional divergence.
Genome-wide identification of the MADS-box transcription factor family in pear (Pyrus bretschneideri) reveals evolution and functional divergence.
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梨 (Pyrus bretschneideri) 中 MADS-box 转录因子家族的全基因组鉴定揭示了进化和功能分歧
作者：
Wang R;Ming M;Li J;Shi D;Qiao X;Li L;Zhang S;Wu J
MADS-box转录因子在植物花器官构象、开花时间和果实发育等发育过程中起着重要作用。梨（Pyrus）作为第三重要的温带水果作物，已经完全测序。然而，关于梨中MADS家族及其功能分化的信息有限。本研究在梨基因组中共鉴定出95个MADS-box基因，并通过系统发育分析将其分为两类。MADS-box型基因分为3个亚家族，II型基因分为14个亚家族。Synteny分析表明，全基因组复制在MADS家族的扩张中发挥了关键作用，随后发生了重排事件。纯化选择是梨MADS-box基因进化的主要驱动力，一对基因在正选择下存在3个密码子位点。通过转录和反转录PCR分析，提供了prmads基因在营养和生殖器官中的完整表达信息。此外，通过双荧光素酶测定，证实了PbrMADS11（12）基因与伙伴PbMYB10和PbbHLH3一起激活了红梨花青素通路结构基因的启动子。此外，我们还推断出PbrMADS11和PbrMADS12参与了花青素合成对光和温度变化的响应调控。这些结果为进一步分析PbrMADS基因在梨色素沉着等不同生物学过程中的功能奠定了基础。
MADS-box transcription factors play significant roles in plant developmental processes such as floral organ conformation, flowering time, and fruit development. Pear (Pyrus), as the third-most crucial temperate fruit crop, has been fully sequenced. However, there is limited information about the MADS family and its functional divergence in pear. In this study, a total of 95 MADS-box genes were identified in the pear genome, and classified into two types by phylogenetic analysis. Type I MADS-box genes were divided into three subfamilies and type II genes into 14 subfamilies. Synteny analysis suggested that whole-genome duplications have played key roles in the expansion of the MADS family, followed by rearrangement events. Purifying selection was the primary force driving MADS-box gene evolution in pear, and one gene pairs presented three codon sites under positive selection. Full-scale expression information for PbrMADS genes in vegetative and reproductive organs was provided and proved by transcriptional and reverse transcription PCR analysis. Furthermore, the PbrMADS11(12) gene, together with partners PbMYB10 and PbbHLH3 was confirmed to activate the promoters of the structural genes in anthocyanin pathway of red pear through dual luciferase assay. In addition, the PbrMADS11 and PbrMADS12 were deduced involving in the regulation of anthocyanin synthesis response to light and temperature changes. These results provide a solid foundation for future functional analysis of PbrMADS genes in different biological processes, especially of pigmentation in pear.
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影响因子：
5.3
作者：
Daminato M;Masiero S;Resentini F;Lovisetto A;Casadoro G
通讯作者：
Casadoro G
大米中的MADS-box基因家族：在生殖发育和压力期间，全基因组识别，组织和表达分析。
影响因子：
4.4
作者：
Arora, Rita;Agarwal, Pinky;Ray, Swatismita;Singh, Ashok Kumar;Singh, Vijay Pal;Tyagi, Akhilesh K.;Kapoor, Sanjay
通讯作者：
Kapoor, Sanjay
影响因子：
7.2
作者：
Dubos, Christian;Le Gourrierec, Jose;Lepiniec, Loic
通讯作者：
Lepiniec, Loic
DOI：
10.1073/pnas.0900906106
发表时间：
2009-04-07
影响因子：
11.1
作者：
Fawcett, Jeffrey A.;Maere, Steven;Van de Peer, Yves
通讯作者：
Van de Peer, Yves
影响因子：
11.4
作者：
Davies, B;EgeaCortines, M;Sommer, H
通讯作者：
Sommer, H