Characterization of TM8, a MADS-box gene expressed in tomato flowers.
Characterization of TM8, a MADS-box gene expressed in tomato flowers.
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TM8的特征是一种用番茄花表达的疯狂盒基因。
DOI：
10.1186/s12870-014-0319-y
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发表时间：
2014-11-30
影响因子：
5.3
通讯作者：
Casadoro G
中科院分区：
文献类型：
--
作者：
Daminato M;Masiero S;Resentini F;Lovisetto A;Casadoro G
花器官的身份是由各种MIKC MADS盒转录因子以组合方式起作用。TM 8是一个MADS盒基因，20多年前从番茄突变体的花分生组织中分离得到，但尽管存在于被子植物和裸子植物中，但从功能的角度来看仍然知之甚少，一些物种含有该基因的多个拷贝。本研究报告了TM 8的表征，进行了在转基因番茄植物与改变表达的基因。制备过表达TM 8或该基因的嵌合阻遏物形式（TM 8：SRDX）的番茄植物。在TM 8上调的植物中，可以观察到异常雄蕊，花粉活力差，几个花身份基因的表达改变，其中包括B-，C-和E-功能基因，而在其他轮中没有明显的形态学修饰。在表达TM 8：SRDX阻遏基因的植株中获得了长圆形子房和单性结实的果实。这样的卵巢显示各种心皮相关基因的修饰表达。在其他的花轮上没有明显的变化。后者也有上位叶和畸形的花分裂区。通过使用酵母双杂交测定，可以表明TM 8能够在酵母中与MACROCALIX相互作用。异位改变的TM 8表达对生殖结构的影响表明，该基因在番茄花的发育中起着一定的作用。MACROCALYX是一个具有A功能的MADS-box基因，在发育完全的花的四个轮生花序中都有表达，在异常雄蕊和子房中的表达量与TM 8相反。由于TM 8蛋白在体外仅与A功能MADS盒蛋白MACROCALYX相互作用，因此似乎对于番茄生殖结构的正确分化，TM 8和MACROCALYX蛋白之间可能的相互作用可能是重要的。本文的在线版本（doi：10.1186/s12870-014-0319-y）包含补充材料，可供授权用户使用。
The identity of flower organs is specified by various MIKC MADS-box transcription factors which act in a combinatorial manner. TM8 is a MADS-box gene that was isolated from the floral meristem of a tomato mutant more than twenty years ago, but is still poorly known from a functional point of view in spite of being present in both Angiosperms and Gymnosperms, with some species harbouring more than one copy of the gene. This study reports a characterization of TM8 that was carried out in transgenic tomato plants with altered expression of the gene. Tomato plants over-expressing either TM8 or a chimeric repressor form of the gene (TM8:SRDX) were prepared. In the TM8 up-regulated plants it was possible to observe anomalous stamens with poorly viable pollen and altered expression of several floral identity genes, among them B-, C- and E-function ones, while no apparent morphological modifications were visible in the other whorls. Oblong ovaries and fruits, that were also parthenocarpic, were obtained in the plants expressing the TM8:SRDX repressor gene. Such ovaries showed modified expression of various carpel-related genes. No apparent modifications could be seen in the other flower whorls. The latter plants had also epinastic leaves and malformed flower abscission zones. By using yeast two hybrid assays it was possible to show that TM8 was able to interact in yeast with MACROCALIX. The impact of the ectopically altered TM8 expression on the reproductive structures suggests that this gene plays some role in the development of the tomato flower. MACROCALYX, a putative A-function MADS-box gene, was expressed in all the four whorls of fully developed flowers, and showed quantitative variations that were opposite to those of TM8 in the anomalous stamens and ovaries. Since the TM8 protein interacted in vitro only with the A-function MADS-box protein MACROCALYX, it seems that for the correct differentiation of the tomato reproductive structures possible interactions between TM8 and MACROCALYX proteins might be important. The online version of this article (doi:10.1186/s12870-014-0319-y) contains supplementary material, which is available to authorized users.
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影响因子：
11.4
作者：
Davies, B;EgeaCortines, M;Sommer, H
通讯作者：
Sommer, H
影响因子：
6.9
作者：
Lin Z;Arciga-Reyes L;Zhong S;Alexander L;Hackett R;Wilson I;Grierson D
通讯作者：
Grierson D
影响因子：
4.9
作者：
Bassa, Carole;Mila, Isabelle;Audran-Delalande, Corinne
通讯作者：
Audran-Delalande, Corinne
影响因子：
6.9
作者：
Chaabouni S;Jones B;Delalande C;Wang H;Li Z;Mila I;Frasse P;Latché A;Pech JC;Bouzayen M
通讯作者：
Bouzayen M
DOI：
10.1007/bf00710588
发表时间：
1951-01-01
期刊：
Zuchter
影响因子：
--
作者：
Helm, J.
通讯作者：
Helm, J.