High-throughput acoustic separation of platelets from whole blood.

High-throughput acoustic separation of platelets from whole blood.
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DOI:
10.1039/c6lc00682e
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发表时间:
2016-09-21
期刊:
影响因子:
6.1
通讯作者:
Huang TJ
Huang TJ
中科院分区:
工程技术1区
文献类型:
--
作者:
Chen Y;Wu M;Ren L;Liu J;Whitley PH;Wang L;Huang TJ

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血小板含有在生物医学和临床应用中重要的生长因子。在这项工作中,我们提出了一种高通量,非侵入性血小板分离的声学分离装置。特别是,我们以10 mL/min的通量从全血中分离血小板,这比现有的基于声学的血小板分离技术大三个数量级。在没有样品稀释的情况下,我们观察到超过80%的RBC/WBC去除和血小板回收。高通量、高分离效率和生物相容性使该装置可用于许多临床应用。一种声学分离装置,其可以实现从未稀释的全血中高通量、高效率和非侵入性的血小板富集。
Platelets contain growth factors which are important in biomedical and clinical applications. In this work, we present an acoustic separation device for high-throughput, non-invasive platelet isolation. In particular, we separated platelets from whole blood at a 10 mL/min throughput, which is three orders of magnitude greater than that of existing acoustic-based platelet separation techniques. Without sample dilution, we observed more than 80% RBC/WBC removal & platelet recovery. High throughput, high separation efficiency, and biocompatibility make this device useful for many clinical applications. An acoustic separation device that can achieve high-throughput, high-efficiency, and non-invasive platelet enrichment from undiluted whole blood.
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