Human eosinophil cationic protein. Molecular cloning of a cytotoxin and helminthotoxin with ribonuclease activity.
Human eosinophil cationic protein. Molecular cloning of a cytotoxin and helminthotoxin with ribonuclease activity.
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人嗜酸性粒细胞阳离子蛋白。具有核糖核酸酶活性的细胞毒素和螺旋毒素的分子克隆。
DOI：
10.1084/jem.170.1.163
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发表时间：
1989-07-01
影响因子：
15.3
通讯作者：
TENEN, DG
中科院分区：
文献类型：
--
作者：
ROSENBERG, HF;ACKERMAN, SJ;TENEN, DG
我们已经分离出一个725 bp的全长cDNA克隆的人嗜酸性粒细胞阳离子蛋白（ECP）。ECP是一种小的碱性蛋白，存在于嗜酸性粒细胞的大特异颗粒基质中，具有细胞毒性、蠕虫毒性和核糖核酸酶活性，是核糖核酸酶多基因家族的成员。cDNA序列显示89%的序列同一性与报道的相关颗粒蛋白，嗜酸性粒细胞衍生的神经毒素（EDN）。开放阅读框架编码一个先前未鉴定的27个氨基酸的前导序列，前导序列位于分子量为15.6 kD的133个残基的成熟ECP多肽之前。ECP编码的氨基酸序列与EDN编码的氨基酸序列有66%的同源性，与人胰腺核糖核酸酶编码的氨基酸序列有31%的同源性，包括基本结构的半胱氨酸、催化赖氨酸和组氨酸残基的保守性。在嗜酸性粒细胞富集的外周粒细胞和用IL-5诱导向嗜酸性粒细胞分化的早幼粒细胞白血病细胞系HL-60的亚克隆中检测到ECP的mRNA。在未诱导的HL-60细胞中，或在用维生素D3诱导向单核细胞分化或用DMSO诱导向嗜酸性细胞分化的HL-60细胞中未检测到ECP mRNA。相反，EDN的mRNA在未诱导的HL-60细胞中被检测到，并且在用DMSO诱导的HL-60细胞中被上调。尽管在序列和细胞定位的相似性，这些结果表明，ECP和EDN受到不同的调节机制。
We have isolated a 725-bp full-length cDNA clone for the human eosinophil cationic protein (ECP). ECP is a small, basic protein found in the matrix of the eosinophil's large specific granule that has cytotoxic, helminthotoxic, and ribonuclease activity, and is a member of the ribonuclease multigene family. The cDNA sequence shows 89% sequence identity with that reported for the related granule protein, eosinophil-derived neurotoxin (EDN). The open reading frame encodes a previously unidentified 27-amino acid leader sequence preceding a 133- residue mature ECP polypeptide with a molecular mass of 15.6 kD. The encoded amino acid sequence of ECP shows 66% identity to that of EDN and 31% identity to that of human pancreatic ribonuclease, including conservation of the essential structural cysteine and cataytic lysine and histidine residues. mRNA for ECP was detected in eosinophil- enriched peripheral granulocytes and in a subclone of the promyelocytic leukemia line, HL-60, induced toward eosinophilic differentiation with IL-5. No ECP mRNA was detected in uninduced HL-60 cells, or in HL-60 cells induced toward monocytic differentiation with vitamin D3 or toward neutrophilic differentiation with DMSO. In contrast, mRNA for EDN was detected in uninduced HL-60 cells and was upregulated in HL-60 cells induced with DMSO. Despite similarities in sequence and cellular localization, these results suggest that ECP and EDN are subject to different regulatory mechanisms.
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DOI：
10.1073/pnas.83.10.3146
发表时间：
1986-05-01
影响因子：
11.1
作者：
GLEICH, GJ;LOEGERING, DA;MCKEAN, DJ
通讯作者：
MCKEAN, DJ
DOI：
10.1016/s0006-291x(86)80310-2
发表时间：
1986-09-30
影响因子：
3.1
作者：
GULLBERG, U;WIDEGREN, B;OLSSON, I
通讯作者：
OLSSON, I
影响因子：
56.9
作者：
HABERMAN.E
通讯作者：
HABERMAN.E
影响因子：
2.9
作者：
FETT, JW;STRYDOM, DJ;VALLEE, BL
通讯作者：
VALLEE, BL
影响因子：
3.4
作者：
DEGRADO, WF;MUSSO, GF;KEZDY, FJ
通讯作者：
KEZDY, FJ