Mass-spectrometry based oxidative lipidomics and lipid imaging: applications in traumatic brain injury.
Mass-spectrometry based oxidative lipidomics and lipid imaging: applications in traumatic brain injury.
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DOI：
10.1111/j.1471-4159.2010.07055.x
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发表时间：
2010-12
影响因子：
4.7
通讯作者：
Bayir H
中科院分区：
文献类型：
--
作者：
Sparvero LJ;Amoscato AA;Kochanek PM;Pitt BR;Kagan VE;Bayir H
脂质，特别是磷脂，是中枢神经系统（CNS）组织结构和功能的基础。磷脂的内源性多不饱和脂肪酸链具有顺式双键，每个双键由一个亚甲基分开。这些磷脂对自由基攻击和氧化修饰非常敏感。分析方法的组合，包括不同版本的色谱法和质谱法允许获得有关脂质及其氧化产物的含量和分布的详细信息，从而构成了新兴的氧化脂质组学领域。越来越明显的是，脂质的特定氧化修饰对许多细胞功能、疾病状态和对氧化应激的反应至关重要。氧化脂质组学开始为创伤性脑损伤（TBI）提供新的机制见解，这可能对急性CNS损伤的治疗方法的开发具有重要的转化潜力。特别是，选择性氧化的一个特定的磷脂，心磷脂，已与损伤的神经元细胞凋亡的启动和进展，从而表明新的药物发现的目标。此外，成像质谱法代表了一个令人兴奋的新的机会，相关的地图的脂质概况及其氧化产物的结构和神经病理学。本文综述了近年来基于质谱和成像质谱的脂质组学和氧化脂质组学在脑外伤磷脂研究中的应用进展。
Lipids, particularly phospholipids, are fundamental to central nervous system (CNS) tissue architecture and function. Endogenous polyunsaturated fatty acid chains of phospholipids possess cis-double bonds each separated by one methylene group. These phospholipids are very susceptible to free-radical attack and oxidative modifications. A combination of analytical methods including different versions of chromatography and mass spectrometry allows obtaining detailed information on the content and distribution of lipids and their oxidation products thus constituting the newly emerging field of oxidative lipidomics. It is becoming evident that specific oxidative modifications of lipids are critical to a number of cellular functions, disease states and responses to oxidative stresses. Oxidative lipidomics is beginning to provide new mechanistic insights into traumatic brain injury (TBI) which may have significant translational potential for development of therapies in acute CNS insults. In particular, selective oxidation of a mitochondria-specific phospholipid, cardiolipin, has been associated with the initiation and progression of apoptosis in injured neurons thus indicating new drug discovery targets. Further, imaging mass-spectrometry represents an exciting new opportunity for correlating maps of lipid profiles and their oxidation products with structure and neuropathology. This review is focused on these most recent advancements in the field of lipidomics and oxidative lipidomics based on the applications of mass-spectrometry and imaging mass-spectrometry as they relate to studies of phospholipids in TBI.
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发表时间：
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