Growth and differentiation of primary and passaged equine bronchial epithelial cells under conventional and air-liquid-interface culture conditions.
Growth and differentiation of primary and passaged equine bronchial epithelial cells under conventional and air-liquid-interface culture conditions.
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DOI：
10.1186/1746-6148-7-26
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发表时间：
2011-06-07
影响因子：
2.6
通讯作者：
Ungemach FR
中科院分区：
文献类型：
--
作者：
Abraham G;Zizzadoro C;Kacza J;Ellenberger C;Abs V;Franke J;Schoon HA;Seeger J;Tesfaigzi Y;Ungemach FR
马会出现类似于人类支气管哮喘的复发性气道阻塞（RAO）。培养中的分化原代马支气管上皮细胞（EBEC）与体内气道细胞密切相似，将有助于研究支气管上皮在气道疾病炎症中的作用。然而，由于 EBEC 培养物的分离和表征受到限制，我们修改和优化了从健康马中生成和培养 EBEC 的技术，以模拟体内条件。通过胰蛋白酶消化获得大量 EBEC，并在有或无血清的情况下成功生长最多 2 代。然而，血清或 ultroser G 被证明对于 ALI 膜插入物上的 EBEC 分化至关重要。在分化时观察到具有基底细胞的假复层粘膜纤毛上皮。此外，与 P0 培养物相比，P1 培养物中的跨上皮阻力 (TEER) 更一致且更高，而 P1 培养物中纤毛形成延迟。这项研究提供了一种获得高产 EBEC 和产生高度分化培养物的有效方法。这些 EBEC 培养物可用于研究紧密连接的形成或识别导致哮喘等肺部疾病的上皮源性炎症因子。
Horses develop recurrent airway obstruction (RAO) that resembles human bronchial asthma. Differentiated primary equine bronchial epithelial cells (EBEC) in culture that closely mimic the airway cells in vivo would be useful to investigate the contribution of bronchial epithelium in inflammation of airway diseases. However, because isolation and characterization of EBEC cultures has been limited, we modified and optimized techniques of generating and culturing EBECs from healthy horses to mimic in vivo conditions. Large numbers of EBEC were obtained by trypsin digestion and successfully grown for up to 2 passages with or without serum. However, serum or ultroser G proved to be essential for EBEC differentiation on membrane inserts at ALI. A pseudo-stratified muco-ciliary epithelium with basal cells was observed at differentiation. Further, transepithelial resistance (TEER) was more consistent and higher in P1 cultures compared to P0 cultures while ciliation was delayed in P1 cultures. This study provides an efficient method for obtaining a high-yield of EBECs and for generating highly differentiated cultures. These EBEC cultures can be used to study the formation of tight junction or to identify epithelial-derived inflammatory factors that contribute to lung diseases such as asthma.
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影响因子：
6
作者：
Lee, MK;Yoo, JW;Roh, HJ
通讯作者：
Roh, HJ
DOI：
10.1016/j.jcf.2004.05.010
发表时间：
2004-08-01
期刊：
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society
影响因子：
--
作者：
Bals, Robert;Beisswenger, Christoph;Chinet, Thierry
通讯作者：
Chinet, Thierry
影响因子：
2.1
作者：
DEJONG, PM;VANSTERKENBURG, MAJA;PONEC, M
通讯作者：
PONEC, M
影响因子：
2.1
作者：
KONDO, M;FINKBEINER, WE;WIDDICOMBE, JH
通讯作者：
WIDDICOMBE, JH
人角蛋白：生物学和病理学。
影响因子：
2.3
作者：
Moll, Roland;Divo, Markus;Langbein, Lutz
通讯作者：
Langbein, Lutz