Phenotypic evolution through variation in splicing of the noncoding RNA COOLAIR.
Phenotypic evolution through variation in splicing of the noncoding RNA COOLAIR.
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DOI：
10.1101/gad.258814.115
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发表时间：
2015-04-01
影响因子：
10.5
通讯作者：
Dean C
中科院分区：
文献类型：
--
作者：
Li P;Tao Z;Dean C
拟南芥在开花过程中的自然变异很大程度上是由于非编码的顺式多态决定了开花基因C(FLC)的不同单倍型。Li等人。发现一个单倍型中的单个自然内含子多态通过特异性地改变FLC反义转录COOLAIR的剪接来影响FLC的表达，从而影响开花。改变的反义剪接通过共转录机制增加FLC的表达，包括封顶FLC新生转录本。非编码序列中的自然多态在多大程度上具有功能后果仍是未知的。拟南芥在开花过程中的自然变异很大程度上是由于非编码的顺式多态决定了开花基因C(FLC)的不同单倍型。在这里，我们证明了一个单倍型中的单个自然内含子多态通过特异性地改变FLC反义转录COOLAIR的剪接来影响FLC的表达，从而影响开花。改变的反义剪接通过共转录机制增加FLC的表达，包括封顶FLC新生转录本。因此，单一的非编码多态可以通过调节非编码转录本而成为表型进化的主要贡献者。
A large proportion of the natural variation in flowering in Arabidopsis thaliana accessions is due to noncoding cis polymorphisms that define distinct haplotypes of FLOWERING LOCUS C (FLC). Li et al. find that a single natural intronic polymorphism in one haplotype affects FLC expression and thus flowering by specifically changing splicing of the FLC antisense transcript COOLAIR. Altered antisense splicing increases FLC expression via a cotranscriptional mechanism involving capping of the FLC nascent transcript. The extent to which natural polymorphisms in noncoding sequences have functional consequences is still unknown. A large proportion of the natural variation in flowering in Arabidopsis thaliana accessions is due to noncoding cis polymorphisms that define distinct haplotypes of FLOWERING LOCUS C (FLC). Here, we show that a single natural intronic polymorphism in one haplotype affects FLC expression and thus flowering by specifically changing splicing of the FLC antisense transcript COOLAIR. Altered antisense splicing increases FLC expression via a cotranscriptional mechanism involving capping of the FLC nascent transcript. Single noncoding polymorphisms can therefore be a major contributor to phenotypic evolution through modulation of noncoding transcripts.
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影响因子：
11.8
作者：
Hornyik, Csaba;Terzi, Lionel C.;Simpson, Gordon G.
通讯作者：
Simpson, Gordon G.
DOI：
10.1073/pnas.1419030111
发表时间：
2014-11-11
影响因子：
11.1
作者：
Csorba, Tibor;Questa, Julia I.;Dean, Caroline
通讯作者：
Dean, Caroline
影响因子：
56.9
作者：
Johanson, U;West, J;Dean, C
通讯作者：
Dean, C
影响因子：
64.5
作者：
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通讯作者：
Choder, Mordechai
影响因子：
56.9
作者：
Coustham, Vincent;Li, Peijin;Dean, Caroline
通讯作者：
Dean, Caroline