Integrative species delimitation in photosynthetic sea slugs reveals twenty candidate species in three nominal taxa studied for drug discovery, plastid symbiosis or biological control.
Integrative species delimitation in photosynthetic sea slugs reveals twenty candidate species in three nominal taxa studied for drug discovery, plastid symbiosis or biological control.
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DOI：
10.1016/j.ympev.2013.07.009
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发表时间：
2013-12
影响因子：
4.1
通讯作者：
Trowbridge, Cynthia D.
中科院分区：
文献类型：
--
作者：
Krug, Patrick J.;Vendetti, Jann E.;Rodriguez, Albert K.;Retana, Jennifer N.;Hirano, Yayoi M.;Trowbridge, Cynthia D.
DNA条形码可以突出传统分类学低估物种丰富度的类群，识别可能对应于未识别物种的线粒体谱系。然而，条形码的关键假设仍然没有测试的许多群体的软体海洋无脊椎动物与解决分类。在这里，我们采用了一种综合的方法来划分物种的食草性海蛞蝓在分支Sacoglossa，其中未被识别的多样性可能会复杂化的药物发现，质体内共生，和生物控制的研究。使用线粒体条形码COI基因和核组蛋白3基因，我们测试了假设，即三个广泛分布的“物种”各自包含一个复杂的独立进化的谱系。形态和生殖特征，然后被用来评估是否每个谱系是可区分的候选物种。“环热带”Elysia ornata包括一个加勒比物种和四个印度-太平洋候选物种，这些物种是抗癌化合物kahalalides的潜在来源。“单型”和高光合作用的Plakobranchus ocellatus，用于研究叶绿体共生超过60年，包括10个候选物种。最后，6个候选物种被区分在Elysia tomentosa复合体，包括潜在的生物控制剂的入侵绿色藻类（蕨藻属）。我们发现，一个候选物种的方法开发的脊椎动物有效地分类在海洋无脊椎动物的神秘多样性，并整合阈值COI距离与非分子特征数据可以划定物种，即使在DNA条形码的共同假设被违反。
DNA barcoding can highlight taxa in which conventional taxonomy underestimates species richness, identifying mitochondrial lineages that may correspond to unrecognized species. However, key assumptions of barcoding remain untested for many groups of soft-bodied marine invertebrates with poorly resolved taxonomy. Here, we applied an integrative approach for species delimitation to herbivorous sea slugs in clade Sacoglossa, in which unrecognized diversity may complicate studies of drug discovery, plastid endosymbiosis, and biological control. Using the mitochondrial barcoding COI gene and the nuclear histone 3 gene, we tested the hypothesis that three widely distributed “species” each comprised a complex of independently evolving lineages. Morphological and reproductive characters were then used to evaluate whether each lineage was distinguishable as a candidate species. The “circumtropical” Elysia ornata comprised a Caribbean species and four Indo-Pacific candidate species that are potential sources of kahalalides, anti-cancer compounds. The “monotypic” and highly photosynthetic Plakobranchus ocellatus, used for over 60 years to study chloroplast symbiosis, comprised 10 candidate species. Finally, six candidate species were distinguished in the Elysia tomentosa complex, including potential biological control agents for invasive green algae (Caulerpa spp.). We show that a candidate species approach developed for vertebrates effectively categorizes cryptic diversity in marine invertebrates, and that integrating threshold COI distances with non-molecular character data can delimit species even when common assumptions of DNA barcoding are violated.
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影响因子：
3.1
作者：
Coquillard, P;Thibaut, T;Coquillard, Y
通讯作者：
Coquillard, Y
影响因子：
3.3
作者：
Edmands, S
通讯作者：
Edmands, S
影响因子：
2.5
作者：
Baumgartner, Finn A.;Motti, Cherie A.;Paul, Nicholas A.
通讯作者：
Paul, Nicholas A.
影响因子：
5.1
作者：
Ashour, Mohamed;Edrada, RuAngelie;Proksch, Peter
通讯作者：
Proksch, Peter
DOI：
10.1098/rstb.2005.1722
发表时间：
2005-10-29
影响因子：
6.3
作者：
DeSalle, R;Egan, MG;Siddall, M
通讯作者：
Siddall, M