WGCNA Analysis Identifies the Hub Genes Related to Heat Stress in Seedling of Rice (Oryza sativa L.)
WGCNA Analysis Identifies the Hub Genes Related to Heat Stress in Seedling of Rice (Oryza sativa L.)
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WGCNA 分析确定了与水稻幼苗热应激相关的核心基因 (Oryza sativa L.)
DOI：
10.3390/genes13061020
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发表时间：
2022-06-06
期刊：
影响因子：
3.5
通讯作者：
Tang, Wenbang
中科院分区：
文献类型：
--
作者：
Wang, Yubo;Wang, Yingfeng;Liu, Xiong;Zhou, Jieqiang;Deng, Huabing;Zhang, Guilian;Xiao, Yunhua;Tang, Wenbang
频繁的高温天气影响水稻的生长发育，造成结实率下降，稻米品质恶化，产量下降。虽然已经克隆了一些耐高温基因，但在解决高温胁迫对水稻的影响方面仍收效甚微。基于7个时间点的转录数据，采用加权相关网络分析（WGCNA）方法构建了耐热胁迫水稻基因型IR64和耐热胁迫水稻基因型Koshihikari差异表达基因（DEGs）的共表达网络。两种基因型中均有4个模块与幼苗热胁迫后的时间点高度相关。我们进一步通过聚类和分析蛋白质与已知核心基因的相互作用网络来确定候选枢纽基因。结果表明，内质网核糖体和蛋白质加工是两种基因型对热胁迫响应的共同途径。淀粉和蔗糖代谢的变化以及次生代谢物的生物合成途径的变化可能是光斑菜对热胁迫敏感的原因。研究结果为了解高温响应机制和培养耐高温材料提供了重要参考。
Frequent high temperature weather affects the growth and development of rice, resulting in the decline of seed–setting rate, deterioration of rice quality and reduction of yield. Although some high temperature tolerance genes have been cloned, there is still little success in solving the effects of high temperature stress in rice (Oryza sativa L.). Based on the transcriptional data of seven time points, the weighted correlation network analysis (WGCNA) method was used to construct a co–expression network of differentially expressed genes (DEGs) between the rice genotypes IR64 (tolerant to heat stress) and Koshihikari (susceptible to heat stress). There were four modules in both genotypes that were highly correlated with the time points after heat stress in the seedling. We further identified candidate hub genes through clustering and analysis of protein interaction network with known–core genes. The results showed that the ribosome and protein processing in the endoplasmic reticulum were the common pathways in response to heat stress between the two genotypes. The changes of starch and sucrose metabolism and the biosynthesis of secondary metabolites pathways are possible reasons for the sensitivity to heat stress for Koshihikari. Our findings provide an important reference for the understanding of high temperature response mechanisms and the cultivation of high temperature resistant materials.
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影响因子：
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