Identification and expression of small multidrug resistance transporters in early-branching anaerobic fungi.
Identification and expression of small multidrug resistance transporters in early-branching anaerobic fungi.
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DOI：
10.1002/pro.4730
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发表时间：
2023-09
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膜包埋转运蛋白赋予细胞基本功能，因为它们介导营养素、废物和效应分子的感知和摄取和排出。混杂的多药输出者与药物和抗生素的耐药性有关，并且与寻求增强细胞工厂菌株对疏水生物产品的耐受性的微生物工程师高度相关。在这里，我们报告了早期分支厌氧真菌（新美鞭毛菌纲）中小多药耐药（SMR）转运蛋白的鉴定。SMR类转运蛋白通常存在于细菌中，但以前在真核生物中尚未报道。在这项研究中，我们表明，SMR转运蛋白从厌氧真菌可以产生异源的模式酵母酿酒酵母，这些蛋白质作为进一步表征的目标的潜力。这些新型厌氧真菌SMR转运蛋白的发现为提高工程微生物菌株的生物生产提供了一条有前途的道路。
Membrane‐embedded transporters impart essential functions to cells as they mediate sensing and the uptake and extrusion of nutrients, waste products, and effector molecules. Promiscuous multidrug exporters are implicated in resistance to drugs and antibiotics and are highly relevant for microbial engineers who seek to enhance the tolerance of cell factory strains to hydrophobic bioproducts. Here, we report on the identification of small multidrug resistance (SMR) transporters in early‐branching anaerobic fungi (Neocallimastigomycetes). The SMR class of transporters is commonly found in bacteria but has not previously been reported in eukaryotes. In this study, we show that SMR transporters from anaerobic fungi can be produced heterologously in the model yeast Saccharomyces cerevisiae, demonstrating the potential of these proteins as targets for further characterization. The discovery of these novel anaerobic fungal SMR transporters offers a promising path forward to enhance bioproduction from engineered microbial strains.
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影响因子：
14.9
作者：
Blum M;Chang HY;Chuguransky S;Grego T;Kandasaamy S;Mitchell A;Nuka G;Paysan-Lafosse T;Qureshi M;Raj S;Richardson L;Salazar GA;Williams L;Bork P;Bridge A;Gough J;Haft DH;Letunic I;Marchler-Bauer A;Mi H;Natale DA;Necci M;Orengo CA;Pandurangan AP;Rivoire C;Sigrist CJA;Sillitoe I;Thanki N;Thomas PD;Tosatto SCE;Wu CH;Bateman A;Finn RD
通讯作者：
Finn RD
影响因子：
14.9
作者：
Grigoriev IV;Nikitin R;Haridas S;Kuo A;Ohm R;Otillar R;Riley R;Salamov A;Zhao X;Korzeniewski F;Smirnova T;Nordberg H;Dubchak I;Shabalov I
通讯作者：
Shabalov I
影响因子：
16.6
作者：
Kermani AA;Macdonald CB;Burata OE;Ben Koff B;Koide A;Denbaum E;Koide S;Stockbridge RB
通讯作者：
Stockbridge RB
影响因子：
11.4
作者：
Jimenez-Bonilla, Pablo;Zhang, Jie;Wang, Yi
通讯作者：
Wang, Yi
影响因子：
4.8
作者：
Lloris-Garcera, Pilar;Bianchi, Frans;von Heijne, Gunnar
通讯作者：
von Heijne, Gunnar