Asymmetry in sexual pheromones is not required for ascomycete mating.
Asymmetry in sexual pheromones is not required for ascomycete mating.
复制标题
DOI：
10.1016/j.cub.2011.06.054
复制
发表时间：
2011-08-23
期刊：
影响因子：
--
通讯作者：
Murray A
中科院分区：
文献类型：
--
作者：
Gonçalves-Sá J;Murray A
我们研究了出芽酵母（Saccharomyces cerevisiae）性别认同的决定因素。高等真菌分为子囊菌和担子菌。大多数子囊菌有两种交配类型：一种（在酵母中称为α，在丝状真菌中称为MAT1-1）产生一个小的，未修饰的肽信息素，另一种（在酵母中称为a，在丝状真菌中称为MAT1-2）产生一个肽信息素结合到C端法尼基，使其非常疏水。在担子菌中，所有的信息素都是脂质修饰的，这是不同门之间的区别特征。我们想知道信息素修饰的不对称性是否是子囊菌成功交配所必需的。我们从丝状子囊菌和担子菌中克隆了受体和信息素基因，并在出芽酵母（Saccharomyces cerevisiae）中表达，以产生新的、可选择的交配对。我们发现，即使两个酵母细胞都分泌a样或α样肽，它们也可以交配。重要的是，无论细胞是否表达a-或α-交配型基因座，除了信息素和信息素受体外，它们还控制着其他性别特异性基因的表达，这都是正确的。我们证明不对称信息素修饰不是子囊菌真菌成功交配所必需的，并证实，在出芽酵母中，交配的主要决定因素是受体及其相应信息素的特异性。
We investigated the determinants of sexual identity in the budding yeast, Saccharomyces cerevisiae. The higher fungi are divided into the Ascomycetes and the Basidiomycetes. Most Ascomycetes have two mating types: one (called α in yeasts and MAT1-1 in filamentous fungi) produces a small, unmodified, peptide pheromone, and the other (a in yeasts and MAT1-2 in filamentous fungi) produces a peptide pheromone conjugated to a C terminal farnesyl group that makes it very hydrophobic. In the Basidiomycetes, all pheromones are lipid-modified, and this difference is a distinguishing feature between the phyla. We asked whether the asymmetry in pheromone modification is required for successful mating in Ascomycetes. We cloned receptor and pheromone genes from a filamentous Ascomycete and a Basidiomycete and expressed these in the budding yeast, Saccharomyces cerevisiae, to generate novel, alternative mating pairs. We find that two yeast cells can mate even when both cells secrete a-like or α-like peptides. Importantly, this is true regardless of whether the cells express the a- or α-mating type loci, which control the expression of other, sex-specific genes, in addition to the pheromones and pheromone receptors. We demonstrate that the asymmetric pheromone modification is not required for successful mating of ascomycete fungi and confirm that, in budding yeast, the primary determinants of mating are the specificity of the receptors and their corresponding pheromones.
登录
查看更多内容
影响因子：
7.8
作者：
Heiman, MG;Walter, P
通讯作者：
Walter, P
影响因子：
5.3
作者：
MARCUS, S;CALDWELL, GA;BECKER, JM
通讯作者：
BECKER, JM
影响因子：
3.3
作者：
Fowler, TJ;DeSimone, SM;Raper, CA
通讯作者：
Raper, CA
影响因子：
5.3
作者：
MCCAFFREY, G;CLAY, FJ;SPRAGUE, GF
通讯作者：
SPRAGUE, GF
影响因子：
11.4
作者：
KUCHLER, K;STERNE, RE;THORNER, J
通讯作者：
THORNER, J