Comparison of multiplex cytokine assays in a pediatric cohort with epilepsy.
Comparison of multiplex cytokine assays in a pediatric cohort with epilepsy.
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癫痫儿童队列中多重细胞因子检测的比较。
DOI：
10.1016/j.heliyon.2021.e06445
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发表时间：
2021-03
期刊：
影响因子：
4
通讯作者：
Di Germanio C
中科院分区：
文献类型：
--
作者：
Numis AL;Fox CH;Lowenstein DJ;Norris PJ;Di Germanio C
多重分析允许在小样品体积中检测数十种细胞因子/趋化因子。虽然有几种市售的检测试剂盒，但在儿科或癫痫队列中没有血浆测量的比较数据。38例癫痫患儿的队列研究。我们使用三种不同的测定法评估细胞因子/趋化因子的血浆水平：Luminex® xMAP高灵敏度（HS）和标准灵敏度（SS）测定法，以及Meso-Scale Discovery（MSD）。我们计算了每种分析物的回收率、测定之间的相关系数和测量之间的一致性水平。我们在单个冻融循环后对样本子集进行了重复分析。在所有分析共有的10种分析物中，HS对所有分析物具有高回收率（<15%的外推值或超出范围[OOR]），SS为50%，MSD为40%。虽然几种分析物在测定之间具有高度相关性，但Bland-Altman图证明测定不可互换。对于大多数分析物，单个冻融循环降低了细胞因子/趋化因子测量值。冻融循环后的测量结果具有良好的相关性，使用HS的13种分析物中有6种（46%）的测量结果之间具有可接受的一致性，SS的9种分析物中有1种（11%）的测量结果之间具有可接受的一致性，MSD的测量结果之间无一致性。HS检测可以优化癫痫研究中特别感兴趣的蛋白质的血浆产量，限制标准曲线外推的值，并与其他SS和MSD检测相比提高精度。我们的研究结果表明，检测方法的选择可能对研究结果至关重要，并支持需要一种标准化的方法来评估癫痫研究和其他领域的生物标志物。儿科癫痫、细胞因子、趋化因子、Luminex、细观发现、神经炎症。
Multiplex analyses allow for detection of dozens of cytokines/chemokines in small sample volumes. Although several commercially available assay kits are available, there are no comparative data in plasma measurements among pediatric or epilepsy cohorts. Cohort study of 38 children with epilepsy. We evaluated plasma levels of cytokines/chemokines using three different assays: Luminex® xMAP high-sensitivity (HS) and standard-sensitivity (SS) assays, and Meso-Scale Discovery (MSD). We calculated recovery rates of each analyte, correlation coefficients between assays, and level of agreement between measurements. We repeated analyses in a subset of samples after a single freeze-thaw cycle. Among ten analytes common to all assays, HS had high recovery (<15% of values extrapolated or out-of- range [OOR]) for all analytes, SS for 50%, and MSD for 40%. While several analytes had a high correlation between assays, Bland-Altman plots demonstrated assays were not interchangeable. For most analytes, a single freeze-thaw cycle decreased cytokines/chemokine measurements. There was good correlation of measurements after a freeze-thaw cycle with acceptable agreement between measurements for six of 13 (46%) analytes using HS, one of 9 (11%) for SS, and none for MSD. HS assays may optimize yield in plasma for proteins of particular interest in epilepsy research, limit values extrapolated beyond the standard curve, and improve precision compared to other SS and MSD assays. Our results demonstrate assay choice may be critical to study results and support the need for a standardized approach to biomarker assessment across epilepsy research and other domains. Pediatric epilepsy, Cytokines, Chemokines, Luminex, Meso-scale discovery, Neuro-inflammation.
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影响因子：
9.3
作者：
Ellington AA;Kullo IJ;Bailey KR;Klee GG
通讯作者：
Klee GG
影响因子：
4.6
作者：
Parkitny L;McAuley JH;Kelly PJ;Di Pietro F;Cameron B;Moseley GL
通讯作者：
Moseley GL
DOI：
10.1007/978-1-62703-520-0_11
发表时间：
2013-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Burguillos, Miguel A
通讯作者：
Burguillos, Miguel A
影响因子：
3.8
作者：
Henno, Linda Torrissen;Storjord, Min;Brekke, Ole-Lars
通讯作者：
Brekke, Ole-Lars
影响因子：
9.3
作者：
Kwon, Young Se;Pineda, Eduardo;Sankar, Raman
通讯作者：
Sankar, Raman