Multiplex cytokine concentration measurement: how much do the medium and handling matter?
Multiplex cytokine concentration measurement: how much do the medium and handling matter?
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DOI：
10.1155/2013/890706
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发表时间：
2013
影响因子：
4.6
通讯作者：
Moseley GL
中科院分区：
文献类型：
--
作者：
Parkitny L;McAuley JH;Kelly PJ;Di Pietro F;Cameron B;Moseley GL
认为细胞因子浓度受采样和处理方法以及储存条件的影响。在这项研究中，我们比较了在基线和受控解冻条件后从血浆和血清中获得的17种细胞因子浓度。我们发现血浆和血清中细胞因子浓度之间的绝对一致性较差，但MIP 1 β除外。解冻条件下显着改变了大多数细胞因子的浓度，但血清受此影响比血浆。使用Bland-Altman分析进行的更仔细检查显示，对于每次比较，许多细胞因子浓度的一致性中等。这一点很重要，因为必须根据所需的精确度来解释一致性指标，而临床和研究需求可能会有所不同。我们还发现，对于某些细胞因子，血清和血浆之间的关系受到浓度的影响，因此主张在系统评价和荟萃分析等研究中进行此类比较时使用适当的方法。
Cytokine concentrations are thought to be affected by methods of sampling and processing and by storage conditions. In this study we compared 17 cytokine concentrations obtained from plasma and serum at baseline and after a controlled thaw condition. We found that absolute agreement was poor between concentrations of cytokines in plasma and serum, except for MIP1β. A thaw condition significantly changed the concentrations of most cytokines, but serum appeared less affected by this than plasma was. Closer examination using Bland-Altman analyses revealed that for each comparison, agreement was moderately good for many cytokine concentrations. This is important because measures of agreement must be interpreted based on the required precision, which may differ between clinical and research demands. We also identified that for some cytokines, the relationship between serum and plasma is affected by concentration, thus advocating for the use of appropriate methods when performing such comparisons in studies such as systematic reviews and meta-analyses.
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