Metabolic routes affecting rubber biosynthesis in Hevea brasiliensis latex.
Metabolic routes affecting rubber biosynthesis in Hevea brasiliensis latex.
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DOI：
10.1093/jxb/err363
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发表时间：
2012-03
影响因子：
6.9
通讯作者：
Wan KL
中科院分区：
文献类型：
--
作者：
Chow KS;Mat-Isa MN;Bahari A;Ghazali AK;Alias H;Mohd-Zainuddin Z;Hoh CC;Wan KL
橡胶树胶乳中的胞液甲氧戊酸(MVA)途径是为顺式聚异戊二烯(橡胶)的生物合成提供异戊烯基二磷酸(IPP)的常规途径。然而，可塑性2-C-甲基-D-赤藓糖醇4-磷酸(MEP)途径可能是IPP的替代来源，因为它是最近在植物中发现的。两条途径的基因在胶乳中的定量RT-PCR(qRT-PCR)表达谱表明，IPP对顺式聚异戊二烯合成的亚细胞区划与类胡萝卜素的合成程度有关。由此，提出了IPP在同一物种内的两种分配和利用方案，即从MEP途径向顺式聚异戊二烯提供的IPP与胶乳中类胡萝卜素的产生有关。随后，对一组乳胶特有的基因转录本进行了测序和组装，然后将它们定位到需要IPP的途径上。包括顺式聚异戊二烯生物合成在内的多达8条这样的途径被确定。我们对IPP前后代谢途径的研究结果构成了以途径知识为导向的方法的一个重要方面，该方法旨在促进转基因橡胶树中顺式聚异戊二烯的生物合成。
The cytosolic mevalonate (MVA) pathway in Hevea brasiliensis latex is the conventionally accepted pathway which provides isopentenyl diphosphate (IPP) for cis-polyisoprene (rubber) biosynthesis. However, the plastidic 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway may be an alternative source of IPP since its more recent discovery in plants. Quantitative RT-PCR (qRT-PCR) expression profiles of genes from both pathways in latex showed that subcellular compartmentalization of IPP for cis-polyisoprene synthesis is related to the degree of plastidic carotenoid synthesis. From this, the occurrence of two schemes of IPP partitioning and utilization within one species is proposed whereby the supply of IPP for cis-polyisoprene from the MEP pathway is related to carotenoid production in latex. Subsequently, a set of latex unique gene transcripts was sequenced and assembled and they were then mapped to IPP-requiring pathways. Up to eight such pathways, including cis-polyisoprene biosynthesis, were identified. Our findings on pre- and post-IPP metabolic routes form an important aspect of a pathway knowledge-driven approach to enhancing cis-polyisoprene biosynthesis in transgenic rubber trees.
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影响因子：
6.9
作者：
Chow, Keng-See;Wan, Kiew-Lian;Yeang, Hoong-Yeet
通讯作者：
Yeang, Hoong-Yeet
影响因子：
5.8
作者：
Conesa, A;Götz, S;Robles, M
通讯作者：
Robles, M
影响因子：
4.8
作者：
Kasahara, H;Hanada, A;Yamaguchi, S
通讯作者：
Yamaguchi, S
DOI：
10.1073/pnas.1031755100
发表时间：
2003-05-27
影响因子：
11.1
作者：
Laule, O;Fürholz, A;Lange, BM
通讯作者：
Lange, BM
影响因子：
4.8
作者：
Oh, SK;Kang, H;Han, KH
通讯作者：
Han, KH