Preharvest long-term exposure to UV-B radiation promotes fruit ripening and modifies stage-specific anthocyanin metabolism in highbush blueberry.
Preharvest long-term exposure to UV-B radiation promotes fruit ripening and modifies stage-specific anthocyanin metabolism in highbush blueberry.
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DOI：
10.1038/s41438-021-00503-4
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发表时间：
2021-04-01
影响因子：
8.7
通讯作者：
Tao R
中科院分区：
文献类型：
--
作者：
Li T;Yamane H;Tao R
紫外线B（UV-B）光（280-315 nm）是调节植物发育和光形态建成的重要环境信号，同时也影响类黄酮途径，包括花青素的生物合成。关于UV-B辐射对水果的影响，短期或采后辐照对水果质量的影响已有充分的文献记载，但长期采前UV-B辐照对水果生长和着色的影响仍不清楚。因此，在这项研究中，我们调查了长期治疗的影响，涉及环境相关的UV-B剂量高丛蓝莓（越桔伞房花序）水果。采前UV-B处理能迅速促进果实生长和糖的积累，这在其它果树品种中是不常见的。UV-B辐射也加速了果实的成熟和着色。双荧光素酶分析证明，在蓝莓中，编码花青素合成关键酶的VcUFGT的表达分别受VcMYBA 1和VcMYBC 2的正调控和负调控。在整个果实发育阶段，UV-B处理上调了VcMYBPA 1的表达，这通过VcMYBA 1增加了VcUFGT的表达。在绿色果期，UV-B处理增加了编码UV受体的HY 5，该受体上调VcMYBPA 1，下调VcMYBC 2，从而促进花青素的积累。另一方面，在成熟果实中，当暴露于UV-B光时，通过HY 5非依赖性途径增加VcMYBC 2水平抑制过量的花青素苷合成。总之，花青素相关的MYB激活剂和抑制剂可以协调平衡蓝莓果实中花青素的积累，UV-B处理可能以阶段特异性的方式影响其效果。本文讨论了采前UV-B处理对改善蓝莓果实品质的潜在效用。
Ultraviolet-B (UV-B) light (280–315 nm) is an important environmental signal that regulates plant development and photomorphogenesis, while also affecting the flavonoid pathway, including anthocyanin biosynthesis. Regarding the effects of UV-B radiation on fruits, the effects of a short-term or postharvest irradiation on fruit quality have been well-documented, but the effects of a long-term preharvest UV-B irradiation on fruit growth and coloration remain unclear. Thus, in this study, we investigated the effects of a long-term treatment involving an environmentally relevant UV-B dose on highbush blueberry (Vaccinium corymbosum) fruit. The preharvest UV-B treatment quickly promoted fruit growth and sugar accumulation, which is not commonly observed in other fruit tree species. The UV-B exposure also accelerated fruit ripening and coloration. The dual-luciferase assay proved that in blueberries, expression of VcUFGT encoding anthocyanin biosynthesis key enzyme, is positively and negatively regulated by VcMYBA1 and VcMYBC2, respectively. Throughout the fruit development stage, the UV-B treatment up-regulated VcMYBPA1 expression, which increased VcUFGT expression via VcMYBA1. In the green fruit stage, the UV-B treatment increased HY5 encoding UV receptor, which up-regulates VcMYBPA1 and down-regulates VcMYBC2, thereby promotes the accumulation of anthocyanins. On the other hand, excessive anthocyanin synthesis was inhibited by increased VcMYBC2 levels in mature fruits when exposed to UV-B light through HY5-independent pathway. In conclusion, anthocyanin-related MYB activators and repressor may coordinately balance the accumulation of anthocyanins in blueberry fruits, with UV-B treatments possibly influencing their effects in a stage-specific manner. The potential utility of preharvest UV-B treatments for improving blueberry fruit quality is discussed herein.
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