Effects of odor stimulation on antidromic spikes in olfactory sensory neurons.
Effects of odor stimulation on antidromic spikes in olfactory sensory neurons.
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气味刺激对嗅觉感觉神经元逆向尖峰的影响。
DOI：
10.1152/jn.90399.2008
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发表时间：
2008
影响因子：
2.5
通讯作者：
Sherrill,Lisa
中科院分区：
文献类型：
--
作者：
Scott,JohnW;Sherrill,Lisa
通过电刺激戊巴比妥麻醉大鼠的嗅球神经层，在大鼠嗅觉感觉神经元 (OSN) 群体中诱发尖峰。这些复合尖峰的潜伏期和记录位置表明它们起源于嗅觉上皮。双同时记录表明 C 纤维范围内的传导速度约为 0.5 m/s。这些尖峰被认为是由逆向激活的嗅觉感觉神经元产生的。使用 5 Hz 的电刺激来跟踪气味反应期间逆向化合物群体峰值的大小和潜伏期的变化。强烈的气味刺激抑制了尖峰的大小并延长了其潜伏期。在长气味刺激过程中潜伏期延长，表明嗅觉受体神经元轴突的持续激活。尖峰抑制量和潜伏期变化与同一位点不同气味剂和不同刺激强度引起的嗅电图 (EOG) 峰值大小密切相关。我们得出的结论是，逆向尖峰抑制曲线合理地表示了气味剂驱动的嗅觉感觉神经元中的尖峰活动，并且峰值尖峰抑制与峰值 EOG 的相关性表明 EOG 作为嗅觉感觉神经元群体细胞内电位估计的准确性。此外，这些结果对嗅觉神经束的交通具有重要意义。我们没有观察到与受刺激和未受刺激的受体神经元相对应的多个峰。这表明嗅觉神经的尖峰同步，可能是通过触觉相互作用。对尖峰潜伏期的长期影响表明，尽管有许多关于嗅觉受体神经元细胞体去极化阻滞的报道，但在整个气味刺激的持续时间内，动作电位在神经中持续存在。最后，强烈的气味刺激几乎完全阻断了逆向尖峰。这表明很大一部分嗅觉轴突被单一的强烈气味刺激激活。
Spikes were evoked in rat olfactory sensory neuron (OSN) populations by electrical stimulation of the olfactory bulb nerve layer in pentobarbital anesthetized rats. The latencies and recording positions for these compound spikes showed that they originated in olfactory epithelium. Dual simultaneous recordings indicated conduction velocities in the C-fiber range, around 0.5 m/s. These spikes are concluded to arise from antidromically activated olfactory sensory neurons. Electrical stimulation at 5 Hz was used to track changes in the size and latency of the antidromic compound population spike during the odor response. Strong odorant stimuli suppressed the spike size and prolonged its latency. The latency was prolonged throughout long odor stimuli, indicating continued activation of olfactory receptor neuron axons. The amounts of spike suppression and latency change were strongly correlated with the electroolfactogram (EOG) peak size evoked at the same site across odorants and across stimulus intensities. We conclude that the curve of antidromic spike suppression gives a reasonable representation of spiking activity in olfactory sensory neurons driven by odorants and that the correlation of peak spike suppression with the peak EOG shows the accuracy of the EOG as an estimate of intracellular potential in the population of olfactory sensory neurons. In addition, these results have important implications about traffic in olfactory nerve bundles. We did not observe multiple peaks corresponding to stimulated and unstimulated receptor neurons. This suggests synchronization of spikes in olfactory nerve, perhaps by ephaptic interactions. The long-lasting effect on spike latency shows that action potentials continue in the nerve throughout the duration of an odor stimulus in spite of many reports of depolarization block in olfactory receptor neuron cell bodies. Finally, strong odor stimulation caused almost complete block of antidromic spikes. This indicates that a very large proportion of olfactory axons was activated by single strong odor stimuli.
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影响因子：
2.9
作者：
ASTIC, L;SAUCIER, D;HOLLEY, A
通讯作者：
HOLLEY, A
影响因子：
5.5
作者：
Reisert, J;Matthews, HR
通讯作者：
Matthews, HR
青蛙的嗅觉粘膜和神经反应。
DOI：
10.1152/ajplegacy.1962.203.2.353
发表时间：
1962
期刊：
The American journal of physiology
影响因子：
--
作者：
M. M. Mozell
通讯作者：
M. M. Mozell
陆龟嗅神经传导速度的活动依赖性变化
DOI：
10.1007/bf00583251
发表时间：
1979
期刊：
Pflügers Archiv
影响因子：
--
作者：
T. Bliss;M. E. Rosenberg
通讯作者：
M. E. Rosenberg
影响因子：
2.9
作者：
D. Trotier;P. Macleod
通讯作者：
P. Macleod