Advances in field-flow fractionation for the analysis of biomolecules: instrument design and hyphenation
Advances in field-flow fractionation for the analysis of biomolecules: instrument design and hyphenation
复制标题
用于生物分子分析的场流分级分离的进展：仪器设计和连字符
DOI：
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发表时间：
2012
影响因子：
4.3
通讯作者：
Wenwan Zhong
中科院分区：
文献类型：
--
作者：
Samantha Schachermeyer;Jonathan Ashby;Wenwan Zhong
场流分离（FFF）通过使用轴向通道流和交叉场来分离分析物。其软分离能力使其成为复杂混合物初始分馏的理想工具，但大洗脱体积和高流速限制了其适用性，无需大量用户操作。仪器和小型化的最新进展已经成功地减小了通道尺寸和洗脱速度，从而减小了每个级分的体积，使得可以方便地将FFF与正交分离技术结合以提高分辨率。通过使用各种分析技术，包括MS，NMR，甚至X射线散射，也可以对FFF产生的馏分进行更详细的分析。这些发展趋势使FFF在分析不同类型分子时具有更强的能力，并将成为FFF技术进一步发展的选择方向。
Field-flow fractionation (FFF) separates analytes by use of an axial channel-flow and a cross-field. Its soft separation capability makes it an ideal tool for initial fractionation of complex mixtures, but large elution volumes and high flow rates have limited its applicability without significant user handling. Recent advances in instrumentation and miniaturization have successfully reduced channel size and elution speed, and thus the volume of each fraction, making it possible to conveniently couple FFF with orthogonal separation techniques for improved resolution. More detailed analysis can also be performed on the fractions generated by FFF by use of diverse analytical techniques, including MS, NMR, and even X-ray scattering. These developmental trends have given FFF more power in the analysis of different types of molecule, and will be the direction of choice for further advances in FFF technology.
影响因子：
3.4
作者：
Huang, Y;Wang, XB;Gascoyne, PRC
通讯作者：
Gascoyne, PRC
影响因子：
3.4
作者：
Yang, J;Huang, Y;Gascoyne, PRC
通讯作者：
Gascoyne, PRC