MicroRNAs play a critical role in tooth development.
MicroRNAs play a critical role in tooth development.
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DOI：
10.1177/0022034510369304
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发表时间：
2010-08
影响因子：
7.6
通讯作者：
Amendt BA
中科院分区：
文献类型：
--
作者：
Cao H;Wang J;Li X;Florez S;Huang Z;Venugopalan SR;Elangovan S;Skobe Z;Margolis HC;Martin JF;Amendt BA
已知微小核糖核酸（MicroRNAs）在许多组织和器官中调节基因功能，但它们在牙齿发育中的表达及功能（如果有的话）尚不清楚。我们试图通过微小核糖核酸筛选分析来识别它们，并通过在牙上皮和间充质中使Dicer1失活来揭示它们的整体作用。与切牙相比，磨牙以及上皮与间充质相比，有不同组的微小核糖核酸表达。在Pitx2 - Cre小鼠的牙上皮中对Dicer1（成熟微小核糖核酸）进行条件性敲除（cKO）会导致出现多颗且有分支的无釉质切牙和无牙尖的磨牙，并改变切牙的形态以及切牙和磨牙的大小和形状。对分化的牙上皮标志物的分析显示成釉细胞分化存在缺陷。相反，在Dicer1条件性敲除中，颈环（干细胞微环境）扩大。这些结果表明，牙齿发育受到微小核糖核酸的严格控制，并且特定的微小核糖核酸调节牙上皮干细胞的分化。
MicroRNAs are known to regulate gene function in many tissues and organs, but their expression and function, if any, in tooth development are elusive. We sought to identify them by microRNA screening analyses and reveal their overall roles by inactivating Dicer1 in the dental epithelium and mesenchyme. Discrete sets of microRNAs are expressed in molars compared with incisors as well as epithelium compared with mesenchyme. Conditional knockout (cKO) of Dicer1 (mature microRNAs) in the dental epithelium of the Pitx2-Cre mouse results in multiple and branched enamel-free incisors and cuspless molars, and change in incisor patterning and in incisor and molar size and shape. Analyses of differentiating dental epithelial markers reveal a defect in amelo-blast differentiation. Conversely, the cervical loop (stem cell niche) is expanded in Dicer1 cKO. These results demonstrate that tooth development is tightly controlled by microRNAs and that specific microRNAs regulate tooth epithelial stem cell differentiation.
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影响因子：
2.7
作者：
Hu, Jan C. -C.;Chun, Yong-Hee P.;Simmer, James P.
通讯作者：
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影响因子：
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影响因子：
30.8
作者：
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通讯作者：
Hannon, GJ