Toxoplasma gondii UBL-UBA Shuttle Protein DSK2s Are Important for Parasite Intracellular Replication.
Toxoplasma gondii UBL-UBA Shuttle Protein DSK2s Are Important for Parasite Intracellular Replication.
复制标题
弓形虫 UBL-UBA 穿梭蛋白 DSK2 对寄生虫细胞内复制很重要
DOI：
10.3390/ijms22157943
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发表时间：
2021-07-26
影响因子：
5.6
通讯作者：
Liu Q
中科院分区：
文献类型：
--
作者：
Zhang H;Yang X;Ying Z;Liu J;Liu Q
弓形虫（T. gondii）是一种重要的人类和兽医病原体，可在免疫功能低下的患者中引起危及生命的疾病。 UBL-UBA 穿梭蛋白家族是泛素-蛋白酶体系统的重要组成部分。在这里，我们鉴定了一种新型 UBL-UBA 穿梭蛋白 DSK2b，其特征在于 N 端泛素样结构域 (UBL) 和 C 端泛素相关结构域 (UBA)。 DSK2b 定位于细胞质和细胞核。 dsk2b 的缺失不会影响泛素化蛋白的降解、体外寄生虫的生长或小鼠体内的毒力。 dsk2b 及其旁系同源物 dsk2a (ΔΔdsk2adsk2b) 的双基因敲除导致泛素化蛋白的显着积累和弓形虫的异步分裂。 ΔΔdsk2adsk2b的生长在体外受到显着抑制，而在小鼠体内的毒力并未减弱。此外，自噬发生在 ΔΔdsk2adsk2b 中，推测它会降解寄生虫中积累的泛素化蛋白。总体而言，DSK2b 是一种新型 UBL-UBA 穿梭蛋白，有助于泛素化蛋白的降解，对于弓形虫的同步细胞分裂非常重要。
Toxoplasma gondii (T. gondii) is an important human and veterinary pathogen causing life-threatening disease in immunocompromised patients. The UBL-UBA shuttle protein family are important components of the ubiquitin–proteasome system. Here, we identified a novel UBL-UBA shuttle protein DSK2b that is charactered by an N-terminal ubiquitin-like domain (UBL) and a C-terminal ubiquitin-associated domain (UBA). DSK2b was localized in the cytoplasm and nucleus. The deletion of dsk2b did not affect the degradation of ubiquitinated proteins, parasite growth in vitro or virulence in mice. The double-gene knockout of dsk2b and its paralogs dsk2a (ΔΔdsk2adsk2b) results in a significant accumulation of ubiquitinated proteins and the asynchronous division of T. gondii. The growth of ΔΔdsk2adsk2b was significantly inhibited in vitro, while virulence in mice was not attenuated. In addition, autophagy occurred in the ΔΔdsk2adsk2b, which was speculated to degrade the accumulated ubiquitinated proteins in the parasites. Overall, DSK2b is a novel UBL-UBA shuttle protein contributing to the degradation of ubiquitinated proteins and is important for the synchronous cell division of T. gondii.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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影响因子：
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通讯作者：
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通讯作者：
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