Cloning and Characterization of ThSHRs and ThSCR Transcription Factors in Taxodium Hybrid 'Zhongshanshan 406'.
Cloning and Characterization of ThSHRs and ThSCR Transcription Factors in Taxodium Hybrid 'Zhongshanshan 406'.
复制标题
作者：
Wang Z;Yin Y;Hua J;Fan W;Yu C;Xuan L;Yu F
在GRAS家族的转录因子中，短根（SHR）和SCARECROW（SCR）是根组织形成的关键调节因子。本研究从红豆杉杂交种“中山山”不定根中分离并鉴定了两个编码SHR蛋白的基因和一个编码SCR蛋白的基因：ThSHR 1（Accession Number MF045148）、ThSHR 2（Accession Number MF045149）和ThSCR（Accession Number MF045152）。基因结构分析表明ThSHR 1、ThSHR 2和ThSCR均为无内含子基因。多个蛋白质序列比对表明，每个相应的蛋白质，ThSHR 1，ThSHR 2和ThSCR，包含五个保守的结构域：亮氨酸七肽重复序列I（LHRI），VHIID基序，亮氨酸七肽重复序列II（LHR II），PFYRE基序，和SAW基序。系统发育分析表明，ThSCR与其他8个物种的SCR蛋白位于SCR进化枝中，而ThSHR 1和ThSHR 2与其他6个物种的SHR蛋白位于SHR进化枝中。这些基因的时间表达模式进行了剖析不定根发育过程中的茎插。而ThSHR 2和ThSCR的表达增加到主根形成之前下降，ThSHR 1的稳定增加，在整个不定根形成。在转基因白杨原生质体中的亚细胞定位研究表明，ThSHR 1，ThSHR 2和ThSCR定位于细胞核。总的来说，这些结果表明，这三个基因编码红豆杉GRAS家族转录因子，和研究结果有助于提高我们的理解SHR和SCR的表达和功能不定根生产过程中，然后可以操纵，以实现高速率的无性繁殖的有价值的树种。
Among the GRAS family of transcription factors, SHORT ROOT (SHR) and SCARECROW (SCR) are key regulators of the formation of root tissues. In this study, we isolated and characterized two genes encoding SHR proteins and one gene encoding an SCR protein: ThSHR1 (Accession Number MF045148), ThSHR2 (Accession Number MF045149) and ThSCR (Accession Number MF045152) in the adventitious roots of Taxodium hybrid ‘Zhongshanshan’. Gene structure analysis indicated that ThSHR1, ThSHR2 and ThSCR are all intron free. Multiple protein sequence alignments showed that each of the corresponding proteins, ThSHR1, ThSHR2 and ThSCR, contained five well-conserved domains: leucine heptad repeat I (LHRI), the VHIID motif, leucine heptad repeat II (LHR II), the PFYRE motif, and the SAW motif. The phylogenetic analysis indicated that ThSCR was positioned in the SCR clade with the SCR proteins from eight other species, while ThSHR1 and ThSHR2 were positioned in the SHR clade with the SHR proteins from six other species. Temporal expression patterns of these genes were profiled during the process of adventitious root development on stem cuttings. Whereas expression of both ThSHR2 and ThSCR increased up to primary root formation before declining, that of ThSHR1 increased steadily throughout adventitious root formation. Subcellular localization studies in transgenic poplar protoplasts revealed that ThSHR1, ThSHR2 and ThSCR were localized in the nucleus. Collectively, these results suggest that the three genes encode Taxodium GRAS family transcription factors, and the findings contribute to improving our understanding of the expression and function of SHR and SCR during adventitious root production, which may then be manipulated to achieve high rates of asexual propagation of valuable tree species.
登录
查看更多内容
影响因子：
7.7
作者：
Clark, Natalie M.;Hinde, Elizabeth;Sozzani, Rosangela
通讯作者：
Sozzani, Rosangela
影响因子：
13.8
作者：
Combet, C;Blanchet, C;Deléage, G
通讯作者：
Deléage, G
影响因子：
4.9
作者：
Miyashima, Shunsuke;Hashimoto, Takashi;Nakajima, Keiji
通讯作者：
Nakajima, Keiji
影响因子：
64.5
作者：
DiLaurenzio, L;WysockaDiller, J;Benfey, PN
通讯作者：
Benfey, PN
影响因子：
14.9
作者：
Sigrist CJ;de Castro E;Cerutti L;Cuche BA;Hulo N;Bridge A;Bougueleret L;Xenarios I
通讯作者：
Xenarios I