Light sensing by opsins and fungal ecology: NOP-1 modulates entry into sexual reproduction in response to environmental cues.
Light sensing by opsins and fungal ecology: NOP-1 modulates entry into sexual reproduction in response to environmental cues.
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DOI：
10.1111/mec.14425
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发表时间：
2018-01
影响因子：
4.9
通讯作者：
Townsend JP
中科院分区：
文献类型：
--
作者：
Wang Z;Wang J;Li N;Li J;Trail F;Dunlap JC;Townsend JP
Understanding the genetic basis of the switch from asexual to sexual lifestyles in response to sometimes rapid environmental changes is one of the major challenges in fungal ecology. Light appears to play a critical role in the switch—but fungal genomes harbor diverse light sensors. Fungal opsins are homologous to bacterial green-light sensory rhodopsins, and their organismal functions in fungi have not been well understood. Three of these opsin-like proteins were widely distributed across fungal genomes, but homologs of the Fusarium opsin-like protein CarO were present only in plant-associated fungi. Key amino acids, including potential retinal binding sites, functionally diverged on the phylogeny of opsins. This diversification of opsin-like proteins could be correlated with life-history associated differences among fungi in their expression and function during morphological development. In N. crassa and related species, knockout of the opsin NOP-1 led to a phenotype in the regulation of the asexual-sexual switch, modulating response to both light and oxygen conditions. Sexual development commenced early in Δnop-1 strains cultured in unsealed plates under constant blue and white light. Furthermore, comparative transcriptomics showed that expression of nop-1 is light-dependent and that the Δnop-1 strain abundantly expresses genes involved in oxidative stress response, genes enriched in NAD/NADP binding sites, genes with functions in proton trans-membrane movement and catalase activity, and genes involved in the homeostasis of protons. Based on these observations, we contend that light and oxidative stress regulate the asexual-sexual switch via light-responsive and ROS pathways in model fungus N. crassa and other fungi.
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DOI：
10.1534/g3.115.020974
发表时间：
2015-10-13
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Cabrera IE;Pacentine IV;Lim A;Guerrero N;Krystofova S;Li L;Michkov AV;Servin JA;Ahrendt SR;Carrillo AJ;Davidson LM;Barsoum AH;Cao J;Castillo R;Chen WC;Dinkchian A;Kim S;Kitada SM;Lai TH;Mach A;Malekyan C;Moua TR;Torres CR;Yamamoto A;Borkovich KA
通讯作者：
Borkovich KA
影响因子：
3.9
作者：
Bielawski, JP;Yang, ZH
通讯作者：
Yang, ZH
影响因子：
11.4
作者：
Chen, Chen-Hui;Ringelberg, Carol S.;Loros, Jennifer J.
通讯作者：
Loros, Jennifer J.
影响因子：
4.8
作者：
Brandt, Sonja;von Stetten, David;Frankenberg-Dinkel, Nicole
通讯作者：
Frankenberg-Dinkel, Nicole
影响因子：
9.2
作者：
Avelar, Gabriela M.;Schumacher, Robert I.;Zaini, Paulo A.;Leonard, Guy;Richards, Thomas A.;Gomes, Suely L.
通讯作者：
Gomes, Suely L.