The F-box protein Rcy1p is involved in endocytic membrane traffic and recycling out of an early endosome in Saccharomyces cerevisiae.
The F-box protein Rcy1p is involved in endocytic membrane traffic and recycling out of an early endosome in Saccharomyces cerevisiae.
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DOI：
10.1083/jcb.149.2.397
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发表时间：
2000-04-17
期刊：
影响因子：
--
通讯作者：
Riezman H
中科院分区：
文献类型：
--
作者：
Wiederkehr A;Avaro S;Prescianotto-Baschong C;Haguenauer-Tsapis R;Riezman H
在酿酒酵母中，内吞物质在到达液泡之前通过不同的膜结合区室运输。在筛选突变体，影响膜运输沿着内吞途径，我们已经确定了一种新的突变体破坏的基因YJL 204 c，我们已改名为RCY 1（回收1）。RCY 1的缺失导致内吞途径在与液泡蛋白分选途径交叉之前的早期阻断。RCY 1的突变导致含有t-SNARE Tlg 1 p的扩大隔室的积累，并靠近细胞扩增区域。此外，内吞标记物，如Ste 2 p和荧光染料，荧光黄和FM 4 -64，被发现在一个类似的扩大隔室后，他们的内化。为了确定rcy 1 Δ是否有再循环缺陷，我们开发了一种测定先前内化的FM 4 -64再循环的方法。这种方法使我们能够真实的跟踪酵母中的循环途径。使用该测定，可以证明膜的再循环在S中是快速的。在一些实施方案中，在酿酒酵母中，主要部分的内化的FM 4 -64在几分钟内分泌回到培养基中。rcy 1 Δ突变体在循环中有很大缺陷。
In Saccharomyces cerevisiae, endocytic material is transported through different membrane-bound compartments before it reaches the vacuole. In a screen for mutants that affect membrane trafficking along the endocytic pathway, we have identified a novel mutant disrupted for the gene YJL204c that we have renamed RCY1 (recycling 1). Deletion of RCY1 leads to an early block in the endocytic pathway before the intersection with the vacuolar protein sorting pathway. Mutation of RCY1 leads to the accumulation of an enlarged compartment that contains the t-SNARE Tlg1p and lies close to areas of cell expansion. In addition, endocytic markers such as Ste2p and the fluorescent dyes, Lucifer yellow and FM4-64, were found in a similar enlarged compartment after their internalization. To determine whether rcy1Δ is defective for recycling, we have developed an assay that measures the recycling of previously internalized FM4-64. This method enables us to follow the recycling pathway in yeast in real time. Using this assay, it could be demonstrated that recycling of membranes is rapid in S. cerevisiae and that a major fraction of internalized FM4-64 is secreted back into the medium within a few minutes. The rcy1Δ mutant is strongly defective in recycling.
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影响因子：
56.9
作者：
Geli, MI;Riezman, H
通讯作者：
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56.9
作者：
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64.5
作者：
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3.5
作者：
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