Expression and modulation of nerve growth factor in murine keratinocytes (PAM 212).
Expression and modulation of nerve growth factor in murine keratinocytes (PAM 212).
复制标题
小鼠角质形成细胞 (PAM 212) 中神经生长因子的表达和调节。
DOI：
10.1172/jci114539
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Sauder,DN
中科院分区：
文献类型：
--
作者：
Tron,VA;Coughlin,MD;Jang,DE;Stanisz,J;Sauder,DN
神经生长因子（NGF）是交感神经和感觉神经系统的正常发育和维持所需的多肽。皮肤已显示含有相对高量的NGF，这与组织中NGF的量与该器官的交感神经支配的程度成比例的发现一致。由于角质形成细胞，皮肤的主要细胞成分，已知产生其他生长因子和细胞因子，我们的实验旨在确定角质形成细胞是否是NGF的来源。来自角化细胞系PAM 212的角化细胞条件培养基含有NGF样活性，约2-3 ng/ml，如通过神经突生长测定所检测的。新鲜分离的BALB/c角质形成细胞含有约0.1 ng/ml。使用针对NGF的cDNA探针，我们证明了PAM 212和BALB/c角质形成细胞中存在1.3 kb的NGF mRNA。由于紫外线辐射（UV）是皮肤细胞因子的一个潜在的重要调节因子，我们研究了UV对NGFmRNA表达的影响。虽然UV最初抑制角质形成细胞NGF mRNA的表达（4小时），到24小时的诱导的NGF mRNA被视为。佛波醇酯也能诱导NGF信号。因此，角质形成细胞合成并表达NGF，其表达受UVB和佛波醇酯调节。图片
Nerve growth factor (NGF) is a polypeptide that is required for normal development and maintenance of the sympathetic and sensory nervous systems. Skin has been shown to contain relatively high amounts of NGF, which is in keeping with the finding that the quantity of NGF in a tissue is proportional to the extent of sympathetic innervation of that organ. Since the keratinocyte, a major cellular constituent of the skin, is known to produce other growth factors and cytokines, our experiments were designed to determine whether keratinocytes are a source of NGF. Keratinocyte-conditioned media from the keratinocyte cell line PAM 212 contained NGF-like activity, approximately 2-3 ng/ml, as detected by the neurite outgrowth assay. Freshly isolated BALB/c keratinocytes contained approximately 0.1 ng/ml. Using a cDNA probe directed against NGF, we demonstrated the presence of a 1.3-kb NGF mRNA in both PAM 212 and BALB/c keratinocytes. Since ultraviolet radiation (UV) is a potentially important modulating factor for cytokines in skin, we examined the effect of UV on NGF mRNA expression. Although UV initially inhibited the expression of keratinocyte NGF mRNA (4 h), by 24 h an induction of NGF mRNA was seen. The NGF signal could also be induced by phorbol esters. Thus, keratinocytes synthesize and express NGF, and its expression is modulated by UVB and phorbol esters.Images
登录
查看更多内容
离解细胞培养物中胚胎小鼠交感神经元的神经生长因子独立发育。
影响因子：
2.7
作者：
M. Coughlin;M. Collins
通讯作者：
M. Collins
影响因子：
64.8
作者：
ULLRICH, A;GRAY, A;DULL, TJ
通讯作者：
DULL, TJ
DOI：
10.1073/pnas.85.14.5282
发表时间：
1988-07
影响因子：
11.1
作者：
M. Peacocke;M. Yaar;C. Mansur;M. Chao;Barbara A. Gilchrest
通讯作者：
M. Peacocke;M. Yaar;C. Mansur;M. Chao;Barbara A. Gilchrest
影响因子：
64.8
作者：
LINDHOLM, D;HEUMANN, R;THOENEN, H
通讯作者：
THOENEN, H
oligo(dT) 与硝酸纤维素上的 RNA 杂交。
DOI：
10.1016/0735-0651(87)90013-6
发表时间：
1987
期刊：
Gene analysis techniques
影响因子：
--
作者：
Calvin B. Harley
通讯作者：
Calvin B. Harley