Analysis of initial laboratory diagnosis of malaria and its accuracy compared with re-testing from 2013 to 2018 in Yunnan Province, China.
Analysis of initial laboratory diagnosis of malaria and its accuracy compared with re-testing from 2013 to 2018 in Yunnan Province, China.
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云南省2013-2018年疟疾实验室初诊分析及与复检准确率比较
DOI：
10.1186/s12936-020-03477-1
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发表时间：
2020-11-12
期刊：
影响因子：
3
通讯作者：
Xue J
中科院分区：
文献类型：
--
作者：
Dong Y;Deng Y;Xu Y;Chen M;Wei C;Zhang C;Mao X;Xue J
根据中国消除疟疾行动计划，县级卫生部门诊断和报告的疟疾病例必须由省级实验室进一步重新确认。云南省疟疾诊断参考实验室(YPMDRL)开始同步实施镜检和巢式聚合酶链式反应(Nest-PCR)检测，以重新检测2013年至2018年云南省县级实验室初步诊断的疟疾病例，并评估YPMDRL与县级实验室鉴定的疟原虫物种的一致性。2013年至2018年，云南省县级实验室完成的疟疾初诊数据每周采集于《中国疾病预防控制信息系统》。对中国疾病预防控制信息系统管理的所有疟疾病例进行了疟原虫镜检和18SRRNA基因套式聚合酶链式反应检测。将复检检测结果反馈给初始诊断和报告单位，以修订疟疾病例类型。2013年至2018年，云南省县级实验室共确诊和报告疟疾病例2869例。复检率为95.6%(2,742/2,869)，2013-2018年复检率上升。2,742例复查标本中，镜检与套式聚合酶链式反应、单纯镜检和套式聚合酶链式反应双重检测的分别为96.7%(2651/2742)、2.2%(59/2742)和1.1%(32/2742)。以YPMDRL诊断为参照，县级实验室疟原虫总符合率为92.6%(2543/2742)。在不一致的199例病例中，经YPMDRL鉴定为阴性病例103例，恶性疟疾45例，间日疟30例，卵形疟11例，疟疾10例。2013年至2018年，云南省中国疾病预防控制信息系统修订登记疟疾病例2747例，其中间日疟2305例，恶性疟421例，卵形疟11例，疟疾10例。通过显微镜和基因检测的双重复检策略提高了云南省疟疾诊断的准确性，基因检测可以可靠地区分疟原虫物种。YPMDRL提供的复检结果为云南省疟疾种类修订提供了权威依据。
According to China’s Malaria Eradication Action Plan, malaria cases diagnosed and reported by health authorities at the county level must be further re-confirmed by provincial laboratories. The Yunnan Province Malaria Diagnostic Reference Laboratory (YPMDRL) began the synchronous implementation of microscopic examinations and nested polymerase chain reaction (nested-PCR) testing to re-test the malaria cases initially diagnosed by county-level laboratories and to evaluate the consistency of Plasmodium species identified between by YPMDRL and by the county-level laboratories from 2013 to 2018 in Yunnan Province. Data on malaria initial diagnosis completed by county-level laboratories in Yunnan Province were collected weekly from the “China Disease Prevention and Control Information System” from 2013 to 2018. The YPMDRL performed Plasmodium microscopic examination and 18S rRNA gene nested-PCR testing on every malaria case managed by the China Disease Prevention and Control Information System. The re-testing detection results were fed back to the initial diagnosis and reporting unit for revision of malaria case types. A total of 2,869 malaria cases were diagnosed and reported by county-level laboratories in Yunnan Province from 2013 to 2018. The re-testing rate was 95.6% (2,742/2,869), and the re-testing rate increased from 2013 to 2018. Among the re-tested 2,742 cases, 96.7% (2651/2742), 2.2% (59/2742), and 1.1% (32/2742) were doubly examined by microscopy and by nested-PCR, only by microscopy, and only by nested-PCR, respectively. The total Plasmodium species accuracy rate at county-level laboratories was 92.6% (2,543/2,742) reference to the diagnosis by YPMDRL. Among the inconsistent 199 cases, they were identified as including 103 negative cases, 45 falciparum malaria cases, 30 vivax malaria cases, 11 ovale malaria cases, and 10 malariae malaria cases by YPMDRL. From 2013 to 2018, the revised and registered malaria cases by the China Disease Prevention and Control Information System in Yunnan Province was 2,747 cases, including 2,305 vivax malaria cases, 421 falciparum malaria cases, 11 ovale malaria cases, and 10 malariae malaria cases. The double re-testing strategy by microscopy and by gene testing increases the accuracy of diagnoses malaria in Yunnan Province, and gene testing can reliably differentiate Plasmodium species. The re-testing results provided by YPMDRL are the authoritative basis for revising malaria kind in Yunnan Province.
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影响因子：
3
作者：
Mueller I;Widmer S;Michel D;Maraga S;McNamara DT;Kiniboro B;Sie A;Smith TA;Zimmerman PA
通讯作者：
Zimmerman PA
影响因子：
3
作者：
Loomans, Laura;Botella, Anali Conesa;Jacobs, Jan
通讯作者：
Jacobs, Jan
影响因子：
2.7
作者：
Sun, Hui;Li, Jin;Wei, Qingkuan
通讯作者：
Wei, Qingkuan
影响因子：
3
作者：
Abanyie FA;Arguin PM;Gutman J
通讯作者：
Gutman J
影响因子：
3
作者：
Kavunga-Membo H;Ilombe G;Masumu J;Matangila J;Imponge J;Manzambi E;Wastenga F;Ngoyi DM;Van Geetruyden JP;Muyembe JJ
通讯作者：
Muyembe JJ