Wheat EARLY FLOWERING 3 affects heading date without disrupting circadian oscillations.
Wheat EARLY FLOWERING 3 affects heading date without disrupting circadian oscillations.
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DOI：
10.1093/plphys/kiac544
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发表时间：
2023-02-12
期刊：
影响因子：
7.4
通讯作者：
Webb, Alex A. R.
中科院分区：
文献类型：
--
作者：
Wittern, Lukas;Steed, Gareth;Taylor, Laura J.;Ramirez, Dora Cano;Pingarron-Cardenas, Gabriela;Gardner, Keith;Greenland, Andy;Hannah, Matthew A.;Webb, Alex A. R.
植物育种者在育种以增加产量和改善作物性能时，已经间接选择了世界上许多主要作物中与昼夜节律相关的基因座的变异。使用一个八亲多亲先进世代间杂交（MAGIC）人口，我们研究了如何在昼夜节律相关基因的变化有助于调节抽穗期在英国和欧洲冬小麦（小麦）品种。在田间条件下，我们鉴定了早熟性本身（Eps）D1和B1基因座的候选基因ERF 3（ELF 3）的同源基因。然后，我们确认了TaELF 3-B1编码区内的单核苷酸多态性作为Eps-B1基因座潜在的候选多态性。我们发现，在Eps-D1基因座包含TaELF 3-D1的报告缺失，而是一个等位基因，位于含有相对于中国春D基因组倒位的渗入区域内。利用小麦（Triticum turgidum cv.）Kronos携带TtELF 3的功能丧失等位基因，我们发现ELF 3调节抽穗，单个ELF 3同源物的丧失足以改变抽穗日期。这些研究表明，ELF 3形成了昼夜节律振荡器的一部分;然而，所有同源物的丢失需要影响昼夜节律。同样，T. aestivum是拟南芥（Arabidopsis thaliana）ELF 3蛋白伴侣的直向同源物，严重扰乱了昼夜节律。ELF 3和LUX转录本在黄昏时不共表达，这表明小麦昼夜节律振荡器的结构可能不同于拟南芥。我们的证明，改变ELF 3 homoeologues可以影响抽穗日期分别从昼夜节律振荡器的影响表明，ELF 3在谷物光周期反应中的作用，可以选择没有多效性的有害改变昼夜节律。谷物的开花时间由昼夜节律振荡器组件调节，该组件与其在昼夜节律系统中的功能分离。
Plant breeders have indirectly selected for variation at circadian-associated loci in many of the world's major crops, when breeding to increase yield and improve crop performance. Using an eight-parent Multiparent Advanced Generation Inter-Cross (MAGIC) population, we investigated how variation in circadian clock-associated genes contributes to the regulation of heading date in UK and European winter wheat (Triticum aestivum) varieties. We identified homoeologues of EARLY FLOWERING 3 (ELF3) as candidates for the Earliness per se (Eps) D1 and B1 loci under field conditions. We then confirmed a single-nucleotide polymorphism within the coding region of TaELF3-B1 as a candidate polymorphism underlying the Eps-B1 locus. We found that a reported deletion at the Eps-D1 locus encompassing TaELF3-D1 is, instead, an allele that lies within an introgression region containing an inversion relative to the Chinese Spring D genome. Using Triticum turgidum cv. Kronos carrying loss-of-function alleles of TtELF3, we showed that ELF3 regulates heading, with loss of a single ELF3 homoeologue sufficient to alter heading date. These studies demonstrated that ELF3 forms part of the circadian oscillator; however, the loss of all homoeologues was required to affect circadian rhythms. Similarly, loss of functional LUX ARRHYTHMO (LUX) in T. aestivum, an orthologue of a protein partner of Arabidopsis (Arabidopsis thaliana) ELF3, severely disrupted circadian rhythms. ELF3 and LUX transcripts are not co-expressed at dusk, suggesting that the structure of the wheat circadian oscillator might differ from that of Arabidopsis. Our demonstration that alterations to ELF3 homoeologues can affect heading date separately from effects on the circadian oscillator suggests a role for ELF3 in cereal photoperiodic responses that could be selected for without pleiotropic deleterious alterations to circadian rhythms. The flowering time of a cereal is regulated by a circadian oscillator component separate from its function in the circadian system.
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影响因子：
18
作者：
Ezer D;Jung JH;Lan H;Biswas S;Gregoire L;Box MS;Charoensawan V;Cortijo S;Lai X;Stöckle D;Zubieta C;Jaeger KE;Wigge PA
通讯作者：
Wigge PA
影响因子：
2.9
作者：
Faricelli, Maria E.;Valarik, Miroslav;Dubcovsky, Jorge
通讯作者：
Dubcovsky, Jorge
影响因子：
5.9
作者：
Brinton J;Ramirez-Gonzalez RH;Simmonds J;Wingen L;Orford S;Griffiths S;10 Wheat Genome Project;Haberer G;Spannagl M;Walkowiak S;Pozniak C;Uauy C
通讯作者：
Uauy C
影响因子：
3.9
作者：
Calixto, Cristiane P. G.;Waugh, Robbie;Brown, John W. S.
通讯作者：
Brown, John W. S.
影响因子：
5.4
作者：
Beales, James;Turner, Adrian;Laurie, David A.
通讯作者：
Laurie, David A.