Control of flowering time and spike development in cereals: the earliness per se Eps-1 region in wheat, rice, and Brachypodium.
Control of flowering time and spike development in cereals: the earliness per se Eps-1 region in wheat, rice, and Brachypodium.
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DOI：
10.1007/s10142-009-0146-7
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发表时间：
2010-05
影响因子：
2.9
通讯作者：
Dubcovsky, Jorge
中科院分区：
文献类型：
--
作者：
Faricelli, Maria E.;Valarik, Miroslav;Dubcovsky, Jorge
二倍体小麦单粒小麦早性基因eps - am1影响抽穗时间、穗发育和小穗数。在本研究中，作为目前克隆该基因工作的一部分，我们比较了来自水稻、黄花蓟马和短茅的Eps1同源区域。一个短茎台BAC克隆跨越了ep -A m1区，但在tauschii的物理图谱中发现了一个缺口。结果表明，小麦与短柄草的同源性高于水稻与短柄草的同源性。然而，大多数结构变化是在小麦系中检测到的。其中包括一个围绕wg241-VatpC区域的反转和六个独特基因的存在。相比之下，在短柄草中只发现一个独特基因（和一个假基因），而在水稻中没有发现。3个基因在短柄草和小麦中均存在，而在水稻中不存在。其中两个基因，Mot1和FtsH4，在单球菌高密度遗传图谱中与早发性表型完全相关，并且是ep - a m1的候选基因。这两个基因都在顶端和发育尖穗中表达，与预期的eps - a1候选基因一致。预测的MOT1蛋白在单球菌亲本系间存在氨基酸差异，但其影响难以预测。未来克隆eps - a1基因的步骤包括mot1和ftsh4突变体的产生，以及完成单球绦虫物理图谱以测试其他候选基因的存在。本文的在线版本（doi:10.1007/s10142-009-0146-7）包含补充材料，可供授权用户使用。
The earliness per se gene Eps-A m 1 from diploid wheat Triticum monococcum affects heading time, spike development, and spikelet number. In this study, the Eps1 orthologous regions from rice, Aegilops tauschii, and Brachypodium distachyon were compared as part of current efforts to clone this gene. A single Brachypodium BAC clone spanned the Eps-A m 1 region, but a gap was detected in the A. tauschii physical map. Sequencing of the Brachypodium and A. tauschii BAC clones revealed three genes shared by the three species, which showed higher identity between wheat and Brachypodium than between them and rice. However, most of the structural changes were detected in the wheat lineage. These included an inversion encompassing the wg241-VatpC region and the presence of six unique genes. In contrast, only one unique gene (and one pseudogene) was found in Brachypodium and none in rice. Three genes were present in both Brachypodium and wheat but were absent in rice. Two of these genes, Mot1 and FtsH4, were completely linked to the earliness per se phenotype in the T. monococcum high-density genetic map and are candidates for Eps-A m 1. Both genes were expressed in apices and developing spikes, as expected for Eps-A m 1 candidates. The predicted MOT1 protein showed amino acid differences between the parental T. monococcum lines, but its effect is difficult to predict. Future steps to clone the Eps-A m 1 gene include the generation of mot1 and ftsh4 mutants and the completion of the T. monococcum physical map to test for the presence of additional candidate genes. The online version of this article (doi:10.1007/s10142-009-0146-7) contains supplementary material, which is available to authorized users.
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影响因子：
5.4
作者：
Bossolini, Eligio;Krattinger, Simon G.;Keller, Beat
通讯作者：
Keller, Beat
DOI：
10.1073/pnas.052397899
发表时间：
2002-03-05
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
2.4
作者：
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通讯作者：
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影响因子：
5.4
作者：
Echenique, V;Stamova, B;Dubcovsky, J
通讯作者：
Dubcovsky, J