Electroacupuncture at Hua Tuo Jia Ji Acupoints Reduced Neuropathic Pain and Increased GABA(A) Receptors in Rat Spinal Cord.
Electroacupuncture at Hua Tuo Jia Ji Acupoints Reduced Neuropathic Pain and Increased GABA(A) Receptors in Rat Spinal Cord.
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DOI：
10.1155/2018/8041820
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Hsieh CL
中科院分区：
文献类型：
--
作者：
Jiang SW;Lin YW;Hsieh CL
慢性缩窄损伤（CCI-）引起的神经性疼痛是临床观察中与痛觉过敏最相似的模型。神经性疼痛是一种在体感觉系统中可能导致自发性疼痛的神经元功能障碍。本研究采用电针（EA）针刺大鼠双侧L4、L6华佗甲集穴（EX-B2）缓解神经性疼痛。18只Sprague-Dawley大鼠随机分为3组：假手术组、2 hz EA组和15 hz EA组。von Frey和冷板试验后，2 hz和15 hz EA组的机械和热痛觉过敏明显低于假手术组。Western blot分析结果显示，三组大鼠背根神经节中γ-氨基丁酸A （GABAA）、腺苷A1受体（A1R）、瞬时受体电位阳离子通道亚家族V成员1 （TRPV1）、TRPV4和代谢性谷氨酸受体3 （mGluR3）表达相似。此外，与假对照组相比，2和15 hz EA组大鼠脊髓中GABAA受体的水平更高。这在A1R、TRPV1、TRPV4或mGluR3受体中没有观察到。此外，上述所有受体在研究大鼠的体感觉皮层中都没有变化，这表明中枢脊髓效应。研究结果为临床应用EA特异性减轻神经性疼痛提供了证据。
Chronic constriction injury- (CCI-) induced neuropathic pain is the most similar model to hyperalgesia in clinical observation. Neuropathic pain is a neuronal dysfunction in the somatosensory system that may lead to spontaneous pain. In this study, electroacupuncture (EA) was applied at bilateral L4 and L6 of Hua Tuo Jia Ji points (EX-B2) for relieving neuropathic pain in rats. Eighteen Sprague-Dawley rats were randomly assigned to three groups: sham, 2-Hz EA, and 15-Hz EA groups. Following von Frey and cold plate tests, both the 2- and the 15-Hz EA groups had significantly lower mechanical and thermal hyperalgesia than the sham group. Western blot analysis results showed that γ-aminobutyric acid A (GABAA), adenosine A1 receptor (A1R), transient receptor potential cation channel subfamily V member 1 (TRPV1), TRPV4, and metabotropic glutamate receptor 3 (mGluR3) were similar in the dorsal root ganglion of all three groups. Furthermore, levels of GABAA receptors were higher in the spinal cord of rats in the 2- and 15-Hz EA groups compared with the sham control group. This was not observed for A1R, TRPV1, TRPV4, or mGluR3 receptors. In addition, all the aforementioned receptors were unchanged in the somatosensory cortex of the study rats, suggesting a central spinal effect. The study results provide evidence to support the clinical use of EA for specifically alleviating neuropathic pain.
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影响因子：
12.4
作者：
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通讯作者：
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