Monitoring Skin Trauma Healing in Mice Using Second-Harmonic Generation Combined with Optical Coherence Tomography
Monitoring Skin Trauma Healing in Mice Using Second-Harmonic Generation Combined with Optical Coherence Tomography
复制标题
使用二次谐波生成结合光学相干断层扫描监测小鼠皮肤创伤愈合
DOI：
10.1109/jphot.2017.2715854
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发表时间：
2017-07
影响因子：
2.4
通讯作者：
Xiaoyuan Deng
中科院分区：
文献类型：
--
作者：
Ning Wang;Huajiang Wei;Ying Jin;Lianzhou Chen;Qilei Zhang;Xiaoyuan Deng
快速评估伤口愈合的简便方法可以帮助临床医生在适当的时间范围内为患者选择有效的治疗措施。在这项研究中，我们探索使用二次谐波产生（SHG）结合光学相干断层扫描（OCT）监测伤口的愈合状态，包括再生组织的宏观形态和光信号特征。在愈合第3、5、7、14、21和28天，使用OCT和SHG对伤口进行成像和监测。采用SHG技术对愈合过程中新生胶原的积累和成熟进行真实的实时检测。此外，使用单散射OCT模型测量创伤前后伤口中OCT信号和衰减系数的变化。伤口闭合百分比、胶原SHG强度和伤口衰减系数使用相关分析进行分析，其产生的相关系数分别为R12 = 0.944、R22 = 0.9776和R32 = 0.9354。从理论上讲，用胶原的SHG强度和衰减系数代替伤口闭合率来评价伤口愈合是可行的。OCT和SHG的结合可以用于真实的诊断伤口愈合。
Convenient methods for rapidly evaluating wound healing could help clinicians to select effective treatment measures for patients within an appropriate time frame. In this study, we explored the use of second-harmonic generation (SHG) combined with optical coherence tomography (OCT) to monitor the healing state of wounds, including the macromorphology and optical signal characteristics of regenerated tissue. Wounds were imaged and monitored using OCT and SHG on healing days 3, 5, 7, 14, 21, and 28. The accumulation and maturation of newly formed collagen during the healing process was detected using SHG in real time. Additionally, changes in the OCT signal and attenuation coefficient in wounds before and after trauma were measured using the single-scattering OCT model. The wound closure percentage, the SHG intensity of collagen, and the attenuation coefficient of the wounds were analyzed using correlation analysis, which produced correlation coefficients of R12 = 0.944, R 22 = 0.9776, and R32 = 0.9354, respectively. In theory, it is feasible to replace the wound closure percentage with the SHG intensity of collagen and the attenuation coefficient to evaluate wound healing. The OCT and SHG combination could be used to diagnose wound healing in real time.
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影响因子：
10.3
作者：
Greaves, N. S.;Benatar, B.;Bayat, A.
通讯作者：
Bayat, A.
影响因子：
3.4
作者：
M. Tsai;Chih-Hsun Yang;S. Shen;Ya-Ju Lee;F. Chang;Cheng-Shin Feng
通讯作者：
M. Tsai;Chih-Hsun Yang;S. Shen;Ya-Ju Lee;F. Chang;Cheng-Shin Feng
影响因子：
14
作者：
Wang, Qiannan;Jin, Ying;Zhan, Zhigang
通讯作者：
Zhan, Zhigang
影响因子：
3.9
作者：
Xue Han;Hanping Liu;Maosheng Chen;Li Gong;Hongwen Pang;Xiaoyuan Deng;Ying Jin
通讯作者：
Xue Han;Hanping Liu;Maosheng Chen;Li Gong;Hongwen Pang;Xiaoyuan Deng;Ying Jin
DOI：
10.1007/s00249-003-0285-z
发表时间：
2003-03
期刊：
European Biophysics Journal
影响因子：
--
作者：
Xiangqun Xu;Ruikang K. Wang;A. E. El Haj
通讯作者：
Xiangqun Xu;Ruikang K. Wang;A. E. El Haj