Pancreas Optical Clearing and 3-D Microscopy in Health and Diabetes.
Pancreas Optical Clearing and 3-D Microscopy in Health and Diabetes.
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DOI：
10.3389/fendo.2021.644826
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发表时间：
2021
影响因子：
5.2
通讯作者：
Tang SC
中科院分区：
文献类型：
--
作者：
Campbell-Thompson M;Tang SC
尽管在一百多年前首次描述，但由于光学清除方法、显微镜系统和三维（3-D）图像分析程序的众多进步，组织光学清除正在经历重新的兴趣。这些进展对于完整的小鼠组织或人体组织块是有利的，因为可以研究几毫米大小的样品。光学透明方法对于中枢和外周神经系统以及组织脉管系统或淋巴系统的神经解剖学的研究特别有用。使用溶剂和水为基础的光学清除方法的例子，小鼠和人类胰腺的结构和网络将在3-D的神经岛复合体，副交感神经节，脂肪细胞浸润以及糖尿病的代谢。光学清除与多重免疫荧光显微镜提供了新的机会，检查神经和循环系统在胰腺和胰岛功能的作用，通过定义他们的神经血管解剖在健康和糖尿病。
Although first described over a hundred years ago, tissue optical clearing is undergoing renewed interest due to numerous advances in optical clearing methods, microscopy systems, and three-dimensional (3-D) image analysis programs. These advances are advantageous for intact mouse tissues or pieces of human tissues because samples sized several millimeters can be studied. Optical clearing methods are particularly useful for studies of the neuroanatomy of the central and peripheral nervous systems and tissue vasculature or lymphatic system. Using examples from solvent- and aqueous-based optical clearing methods, the mouse and human pancreatic structures and networks will be reviewed in 3-D for neuro-insular complexes, parasympathetic ganglia, and adipocyte infiltration as well as lymphatics in diabetes. Optical clearing with multiplex immunofluorescence microscopy provides new opportunities to examine the role of the nervous and circulatory systems in pancreatic and islet functions by defining their neurovascular anatomy in health and diabetes.
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影响因子：
29
作者：
Coate KC;Cha J;Shrestha S;Wang W;Gonçalves LM;Almaça J;Kapp ME;Fasolino M;Morgan A;Dai C;Saunders DC;Bottino R;Aramandla R;Jenkins R;Stein R;Kaestner KH;Vahedi G;HPAP Consortium;Brissova M;Powers AC
通讯作者：
Powers AC
影响因子：
2.9
作者：
Abbruzzese JL;Andersen DK;Borrebaeck CAK;Chari ST;Costello E;Cruz-Monserrate Z;Eibl G;Engleman EG;Fisher WE;Habtezion A;Kim SK;Korc M;Logsdon C;Lyssiotis CA;Pandol SJ;Rustgi A;Wolfe BM;Zheng L;Powers AC
通讯作者：
Powers AC
影响因子：
48
作者：
Chung, Kwanghun;Deisseroth, Karl
通讯作者：
Deisseroth, Karl
影响因子：
8.2
作者：
Chiu, Y. -C.;Hua, T. -E.;Tang, S. -C.
通讯作者：
Tang, S. -C.
影响因子：
1.2
作者：
Campbell-Thompson, Martha L.;Montgomery, Emily L.;Atkinson, Mark A.
通讯作者：
Atkinson, Mark A.