Glial and neuronal glutamate transport following glutamine synthetase inhibition.
Glial and neuronal glutamate transport following glutamine synthetase inhibition.
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谷氨酰胺合成酶抑制后的神经胶质和神经元谷氨酸转运。
DOI：
10.1016/0006-2952(85)90102-9
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发表时间：
1985
影响因子：
5.8
通讯作者：
Tabakoff,B
中科院分区：
文献类型：
--
作者：
Rothstein,JD;Tabakoff,B
用甲硫氨酸亚砜亚胺（MSO）抑制谷氨酰胺合成酶后，研究了谷氨酸转运到纹状体组织中的情况。纹状体组织棱柱中的谷氨酸摄取在脑室内（i.c.v.）注射MSO。谷氨酸摄取的动力学分析表明，高和低亲和力的载体系统介导的谷氨酸运输到组织切片。MSO改变了谷氨酸通过高亲和力载体的转运，而不改变低亲和力谷氨酸转运的特征。MSO增加了谷氨酸的Km和高亲和力摄取位点的Vmax。谷氨酸摄取的Km和Vmax在注射MSO后24小时变化最大，但转运系统在注射后14天恢复正常。此外，MSO增加了组织切片中高亲和力天冬氨酸的摄取，但对亮氨酸的摄取没有影响。谷氨酸摄取到纹状体突触体和散装隔离的胶质细胞或神经元，在所有情况下，介导的低和高亲和力载体。高亲和力胶质细胞谷氨酸摄取的KmandVmax值增加24小时后，i.c.v.注射MSO，而胶质细胞谷氨酸摄取的低亲和力动力学参数没有改变MSO。注射后24小时，MSO既不改变高亲和力也不改变低亲和力谷氨酸摄取到批量分离的神经元或突触体。这些结果表明，MSO诱导的纹状体切片内谷氨酸转运的改变可能是由于谷氨酸代谢中断引起的神经胶质谷氨酸转运的变化。
Glutamate transport into striatal tissue preparations was studied following inhibition of glutamine synthetase with methionine sulfoximine (MSO). Glutamate uptake in striatal tissue prisms was elevated for up to 7 days following an intraventricular (i.c.v.) injection of MSO. Kinetic analysis of glutamate uptake revealed that a high- and a low-affinity carrier system mediated the transport of glutamate into tissue slices. MSO altered the transport of glutamate via the high-affinity carrier without changing the characteristics of low-affinity glutamate transport. MSO increased theKm, for glutamate and theVmaxat the high-affinity uptake site. The changes in the Kmand theVmaxfor glutamate uptake were maximal 24 hr after administration of MSO, but the transport system returned to normal by 14 days after injection. In addition, MSO increased high-affinity aspartate uptake into tissue slices, but it was without effect on leucine uptake. Glutamate uptake into striatal synaptosomes and bulk-isolated glial cells or neurons was, in all cases, mediated by a low- and high-affinity carrier. TheKmandVmaxvalues for high-affinity glial-glutamate uptake were increased 24 hr after i.c.v. injection of MSO, while the low-affinity kinetic parameters for glial glutamate uptake were not altered by MSO. Neither highaffinity nor low-affinity glutamate uptake into bulk-isolated neurons or synaptosomes was altered by MSO 24 hr after injection. These results suggest that MSO induced alterations in glutamate transport within striatal slices may be due to changes in glial glutamate transport arising from the disruption of glutamate metabolism.
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大鼠脑体外 [3H] 多巴胺的区域释放：阿片类药物对钾、尼古丁和 L-谷氨酸诱导的释放的影响。
影响因子：
2.1
作者：
M. Marien;J. Brien;K. Jhamandas
通讯作者：
K. Jhamandas
高亲和力大鼠皮质突触体转运系统对天冬氨酸的净摄取
影响因子：
2.9
作者：
R. Roskoski
通讯作者：
R. Roskoski
类似物的抑制模式表明脑细胞中存在十个或更多氨基酸运输系统
影响因子：
4.7
作者：
H. Sershen;A. Lajtha
通讯作者：
A. Lajtha
神经元和神经胶质细胞以及亚细胞成分的分离
DOI：
10.1016/b978-0-12-008301-5.50015-7
发表时间：
1980
期刊：
Advances in Cell Biology
影响因子：
--
作者：
F. Henn
通讯作者：
F. Henn
影响因子：
2.9
作者：
RONZIO, RA;ROWE, WB;MEISTER, A
通讯作者：
MEISTER, A