A general strategy for the chemoenzymatic synthesis of asymmetrically branched N-glycans.
A general strategy for the chemoenzymatic synthesis of asymmetrically branched N-glycans.
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DOI：
10.1126/science.1236231
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发表时间：
2013-07-26
期刊：
影响因子：
--
通讯作者：
Boons GJ
中科院分区：
文献类型：
--
作者：
Wang Z;Chinoy ZS;Ambre SG;Peng W;McBride R;de Vries RP;Glushka J;Paulson JC;Boons GJ
研究聚糖结合蛋白的特异性和生物学特性需要一种系统的、有效的方法来产生不同的不对称分支N-聚糖文库。为此，我们描述了一种核心五糖，其在潜在的分支位置处通过正交保护基团进行修饰，以允许通过化学糖基化选择性连接独特的糖部分。以这样的方式选择的附件，所得到的脱保护的化合物的触角可以选择性地延长由糖基转移酶，以得到不对称的多触角聚糖库。通过制备一系列复合寡糖来证明该方法的功效，所述复合寡糖被打印为微阵列并筛选与凝集素和流感病毒血凝素的结合，这表明在复合N-聚糖的背景下，通过呈递最小表位来调节识别。
A systematic, efficient means of producing diverse libraries of asymmetrically branched N-glycans is needed to investigate the specificities and biology of glycan binding proteins. To that end, we describe a core pentasaccharide that at potential branching positions is modified by orthogonal protecting groups to allow selective attachment of unique saccharide moieties by chemical glycosylation. The appendages were selected in such a way that the antenna of the resulting deprotected compounds could be selectively extended by glycosyltransferases to give libraries of asymmetrical multi-antennary glycans. The power of the methodology was demonstrated by the preparation of a series of complex oligosaccharides that were printed as microarrays and screened for binding to lectins and influenza-virus hemagglutinins, which showed that recognition is modulated by presentation of minimal epitopes in the context of complex N-glycans.
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影响因子：
4.3
作者：
Serna, Sonia;Etxebarria, Juan;Reichardt, Niels-Christian
通讯作者：
Reichardt, Niels-Christian
影响因子：
3.7
作者：
Chen LM;Blixt O;Stevens J;Lipatov AS;Davis CT;Collins BE;Cox NJ;Paulson JC;Donis RO
通讯作者：
Donis RO
影响因子：
4.3
作者：
Sun, Bin;Srinivasan, Balasubramanian;Huang, Xuefei
通讯作者：
Huang, Xuefei
影响因子：
4.3
作者：
Bohorov, Ognian;Andersson-Sand, Hillevi;Blixt, Ola
通讯作者：
Blixt, Ola
影响因子：
5.9
作者：
Imai M;Kawaoka Y
通讯作者：
Kawaoka Y