Mapping the stem cell state: eight novel human embryonic stem and embryonal carcinoma cell antibodies.
Mapping the stem cell state: eight novel human embryonic stem and embryonal carcinoma cell antibodies.
复制标题
绘制干细胞状态：八种新型人类胚胎干细胞和胚胎癌细胞抗体。
DOI：
10.1111/j.1365-2605.2011.01185.x
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发表时间：
2011
影响因子：
--
通讯作者：
Wright A
中科院分区：
文献类型：
--
作者：
Wright A
人胚胎干 (ES) 和胚胎癌细胞 (EC) 细胞的抗原谱已成为其表征的关键要素，目前广泛使用一组通用的表面和细胞内标记。此类标记已用于识别“未分化状态”内的细胞，但这种分类似乎过于简单化，因为该状态中似乎存在许多子状态。为了提高未分化状态的分辨率，我们生成了八种新型单克隆抗体，它们都能够识别未分化的人类 ES 和 EC 细胞，并在本文中描述了它们的特征。报道了这些抗体针对一系列细胞系的反应性，以及它们的发育调节、基本生物化学和睾丸生殖细胞肿瘤的免疫组织化学反应性。我们的数据揭示了针对 ES 和 EC 细胞的所有抗体的一系列反应性，表明这些标记将提供对未分化干细胞区室中独特亚状态的识别。
The antigenic profile of human embryonic stem (ES) and embryonal carcinoma (EC) cells has served as a key element of their characterization, with a common panel of surface and intracellular markers now widely used. Such markers have been used to identify cells within the ‘undifferentiated state’, yet it appears that this categorization may be an oversimplification, because a number of sub‐states appear to exist within this state. To increase the resolution of the undifferentiated state, we have generated eight novel monoclonal antibodies, all capable of recognizing undifferentiated human ES and EC cells, and herein describe their characterization. The reactivity of these antibodies against a range of cell lines is reported, as well as their developmental regulation, basic biochemistry and reactivity in immunohistochemistry of testicular germ cell tumours. Our data reveal a range of reactivity for all antibodies against both ES and EC cells, suggesting that these markers will afford recognition of unique sub‐states within the undifferentiated stem cell compartment.
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影响因子：
3.5
作者：
Enver, T;Soneji, S;Andrews, PW
通讯作者：
Andrews, PW
影响因子：
2.7
作者：
FENDERSON, BA;ANDREWS, PW;HAKOMORI, SI
通讯作者：
HAKOMORI, SI
影响因子：
--
作者：
M. Pera;A. Filipczyk;S. Hawes;A. Laslett
通讯作者：
A. Laslett
影响因子：
23.4
作者：
B. Fenderson;N. Radin;P. Andrews
通讯作者：
B. Fenderson;N. Radin;P. Andrews
用于制造分泌特异性抗体的杂交瘤的更好细胞系
影响因子：
64.8
作者：
M. Shulman;C. Wilde;G. Köhler
通讯作者：
G. Köhler