Colorimetric Detection of Lead Ion Based on Gold Nanoparticles and Lead-Stabilized G-Quartet Formation

Colorimetric Detection of Lead Ion Based on Gold Nanoparticles and Lead-Stabilized G-Quartet Formation
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基于金纳米粒子和铅稳定的 G-四重体形成的铅离子比色检测

DOI:
10.4236/jbise.2015.87042
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发表时间:
2015-07
期刊:
J. Biomedical Science and Engineering
影响因子:
--
通讯作者:
Shixiong Deng
Shixiong Deng
中科院分区:
其他
文献类型:
--
作者:
Ruiying Zhang;Qifeng Jiang;Xingliang Xiong;Shixiong Deng

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基于单链DNA和G-四重链在金纳米粒子表面的不同吸附能力,我们提出了一种检测Pb2+的方法。在没有Pb2+的情况下,DNA寡核苷酸探针可以被吸附在AuNPs的表面,保护它们不被聚集。加入Pb2+后,DNA寡核苷酸探针可以特异性地形成致密的G-四联体,从而诱导未修饰的AuNPs聚集,特别是在加入氯化钠水溶液后。因此,颜色从红色变为蓝色。通过金纳米粒子的比色反应可以检测到Pb2+,其检出限可达5μM,仅用肉眼即可观察到。大多数金属离子无干扰,加入半胱氨酸可有效消除Cu2+的干扰。为Pb2+的现场实时检测提供了一种简单有效的比色传感器。
In this report, we present a method for the detection of Pb2+ based on the different adsorption capacity on the surface of gold nanoparticles (AuNPs) between ssDNA (single-stranded DNA) and G-quartet. In the absence of Pb2+, the DNA oligonucleotides probe, which is guanine-rich ssDNA, can be adsorbed on the surface of AuNPs protecting them from aggregation. After adding Pb2+, the DNA oligonucleotides probe can specifically form compact G-quartet, which can induce the aggregation of unmodified AuNPs, especially after adding NaCl aqueous solution. Consequently, the color turns from red to blue. Pb2+ can be detected by colorimetric response of AuNPs; its detection limit can reach 5 μM only observed by naked eyes. Most metal ions have no interferences, and the interference of Cu2+ can be effectively eliminated by adding cysteine. It provides a simple and effective colorimetric sensor for on-site and real time detection of Pb2+.
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