Investigation of early cell–surface interactions of human mesenchymal stem cells on nanopatterned β-type titanium–niobium alloy surfaces
Investigation of early cell–surface interactions of human mesenchymal stem cells on nanopatterned β-type titanium–niobium alloy surfaces
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纳米图案β型钛铌合金表面人间充质干细胞早期细胞表面相互作用的研究
DOI：
10.1098/rsfs.2013.0046
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发表时间：
2014
期刊：
影响因子：
4.4
通讯作者：
Cavalcanti-Adam EA
中科院分区：
文献类型：
--
作者：
Medda R;Helth A ;Herre P;Pohl D;Rellinghaus B;Perschmann N;Neubauer S;Kessler H;Oswald S;Eckert J;Spatz JP;Gebert A;Cavalcanti-Adam EA
源自骨髓的多能成体间充质干细胞（MSC）具有治疗骨疾病和再生医学的潜力。然而，它们表型的内在异质性进而导致不同的分化潜能，使得预测这些细胞的反应变得困难。本研究的目的是研究改良钛合金上人类 MSC 的初始细胞与表面相互作用。通过嵌段共聚物胶束纳米光刻将金纳米颗粒沉积在 β 型 Ti-40Nb 合金上，作为纳米形貌线索以及固定存在于几种细胞外基质蛋白中的硫醇肽的特异性结合位点。抛光和纳米图案的 Ti-40Nb 样品中仍然存在 MSC 异质性。然而，用环状 RGD 肽对金纳米粒子进行功能化后，细胞异质性和供体变异性降低。特别是，由于细胞锚定位点的排列，而不是肽特异性，24小时后大细胞的数量显着减少。然而，与对照肽（cRADfK）相比，在整合素结合肽（cRGDfK）存在的情况下，整合素介导的粘附簇的大小和数量增加。这些结果表明，以明确的模式使用整合素配体可以改善间充质干细胞-材料的相互作用，不仅可以通过局部调节细胞粘附，还可以通过减少群体异质性。
Multi-potent adult mesenchymal stem cells (MSCs) derived from bone marrow have therapeutic potential for bone diseases and regenerative medicine. However, an intrinsic heterogeneity in their phenotype, which in turn results in various differentiation potentials, makes it difficult to predict the response of these cells. The aim of this study is to investigate initial cell–surface interactions of human MSCs on modified titanium alloys. Gold nanoparticles deposited on β-type Ti–40Nb alloys by block copolymer micelle nanolithography served as nanotopographical cues as well as specific binding sites for the immobilization of thiolated peptides present in several extracellular matrix proteins. MSC heterogeneity persists on polished and nanopatterned Ti–40Nb samples. However, cell heterogeneity and donor variability decreased upon functionalization of the gold nanoparticles with cyclic RGD peptides. In particular, the number of large cells significantly decreased after 24 h owing to the arrangement of cell anchorage sites, rather than peptide specificity. However, the size and number of integrin-mediated adhesion clusters increased in the presence of the integrin-binding peptide (cRGDfK) compared with the control peptide (cRADfK). These results suggest that the use of integrin ligands in defined patterns could improve MSC-material interactions, not only by regulating cell adhesion locally, but also by reducing population heterogeneity.
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DOI：
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发表时间：
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Journal of materials science. Materials in medicine
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