The involvement of TLR2 and TLR4 in cytokine and nitric oxide production in visceral leishmaniasis patients before and after treatment with anti-leishmanial drugs.
The involvement of TLR2 and TLR4 in cytokine and nitric oxide production in visceral leishmaniasis patients before and after treatment with anti-leishmanial drugs.
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DOI：
10.1371/journal.pone.0117977
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Calvi SA
中科院分区：
文献类型：
--
作者：
Gatto M;de Abreu MM;Tasca KI;de Assis Golim M;da Silva LD;Simão JC;Fortaleza CM;de Campos Soares ÂM;Calvi SA
尽管内脏利什曼病(VL)患者接受抗利什曼药物治疗前后Toll样受体(Toll样受体)、细胞因子与一氧化氮(NO)之间的关系尚不清楚，但Toll样受体(TLRs)与利什曼原虫感染密切相关。本研究旨在探讨系统性红斑狼疮患者抗利什曼药物治疗前后外周血单个核细胞产生肿瘤坏死因子-α、干扰素-γ、IL-17、转化生长因子-β和一氧化氮的变化。此外，我们还研究了这些受体是否参与了这些细胞因子和NO的产生。活动期VL患者淋巴细胞和单核细胞TLR2、TLR4表达增加，肿瘤坏死因子-α、IL-10和转化生长因子-β的产生增加，干扰素-γ、IL-17和NO的产生减少。治疗后T淋巴细胞和单核细胞仍表达TLR2和TLR4，α和IL-10水平降低，干扰素-γ、IL-17和NO水平升高，转化生长因子-β水平持续升高。治疗前肿瘤坏死因子-α和一氧化氮的产生与TLR2和TLR4的表达相关，而IL-10的产生仅与TLR2的表达相关。治疗后，两种受体均与肿瘤坏死因子-α、干扰素-γ、IL-10和NO的产生有关，而IL-17的产生仅与TLR4的表达有关。本研究结果提示，TLR2和TLR4均参与了VL患者细胞因子和NO的调节，参与了VL治疗前的发病机制和治疗后的保护性免疫反应。
Toll-like receptors (TLRs) have significant involvement in Leishmania infection, although little is known about the relationship between these receptors, cytokines and nitric oxide (NO) in patients with visceral leishmaniasis (VL) before or after treatment with anti-leishmanial drugs. The goal of this study was to evaluate the expression of TLR2 and TLR4 in CD3+ and CD14+ cells and the production of TNF-α, IFN-γ, IL-17, IL-10, TGF-β and NO in peripheral blood mononuclear cells (PBMCs) from VL patients pre- and post-treatment with anti-leishmanial drugs. In addition, we investigated whether these receptors were involved in the production of these cytokines and NO. In the active VL patients, increased TLR2 and TLR4 expression in lymphocytes and monocytes, increased production of TNF-α, IL-10 and TGF-β and decreased production of IFN-γ, IL-17 and NO were observed. After treatment, TLR2 and TLR4 were still expressed in lymphocytes and monocytes, the TNF-α and IL-10 levels were lower, the production of IFN-γ, IL-17 and NO was higher, and the TGF-β level remained high. Before treatment, the production of TNF-α and NO was associated with TLR2 and TLR4 expression, while IL-10 production was only associated with TLR2 expression. After treatment, both receptors were associated with the production of TNF-α, IFN-γ, IL-10 and NO, while the production of IL-17 was associated only with TLR4 expression. The results presented in this study suggest that both TLR2 and TLR4 participate in the modulation of cytokine and NO production in VL patients, contributing to the pathogenesis of VL prior to treatment and the protective immune response after treatment.
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影响因子：
8.6
作者：
Ansari, Nasim Akhtar;Saluja, Sumita;Salotra, Poonam
通讯作者：
Salotra, Poonam
白介素（IL）-10在愈合后皮肤中持续的持久性以及抗IL-10受体抗体对无菌治疗的治疗潜力的作用。
DOI：
10.1084/jem.194.10.1497
发表时间：
2001-11-19
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Belkaid Y;Hoffmann KF;Mendez S;Kamhawi S;Udey MC;Wynn TA;Sacks DL
通讯作者：
Sacks DL
影响因子：
--
作者：
Abdollahi-Roodsaz, Shahla;Joosten, Leo A. B.;van den Berg, Wim B.
通讯作者：
van den Berg, Wim B.
DOI：
10.1590/s0074-02762012000600005
发表时间：
2012-09-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Costa, Alinne Silva Andrade;Costa, Graciomar Conceição;Caldas, Arlene de Jesus Mendes
通讯作者：
Caldas, Arlene de Jesus Mendes
影响因子：
5.4
作者：
Flandin, JF;Chano, F;Descoteaux, A
通讯作者：
Descoteaux, A