The neuroanatomical distribution of oxytocin receptor binding and mRNA in the male rhesus macaque (Macaca mulatta).
The neuroanatomical distribution of oxytocin receptor binding and mRNA in the male rhesus macaque (Macaca mulatta).
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DOI：
10.1016/j.psyneuen.2014.03.023
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发表时间：
2014-07
影响因子：
3.7
通讯作者：
Young, Larry J.
中科院分区：
文献类型：
--
作者：
Freeman, Sara M.;Inoue, Kiyoshi;Smith, Aaron L.;Goodman, Mark M.;Young, Larry J.
恒河猴（Macaca mulatta）是社会认知的重要灵长类动物模型，最近的研究开始探讨催产素对社会认知和行为的影响。猕猴在阐明催产素调节社会认知的神经机制方面具有巨大潜力，这对基于催产素的治疗自闭症和精神分裂症等精神疾病的药物疗法具有重要意义。先前在恒河猴大脑中定位催产素受体（OXTR）的尝试因放射性配体的选择性降低而失败，在灵长类动物中，放射性配体与 OXTR 和结构相似的加压素 1a 受体（AVPR1A）结合。我们开发了一种基于药理学的竞争性结合放射自显影方案，可选择性地揭示灵长类动物脑切片中的 OXTR 和 AVPR1A 结合位点。使用该协议，我们描述了猕猴中 OXTR 的神经解剖学分布。最后，我们使用原位杂交来定位 OXTR mRNA。我们的结果表明，猕猴大脑中的 OXTR 表达比 AVPR1A 受到更多限制。 OXTR主要局限于Meynert基底核、桥脚被盖核、上丘浅灰层、梯形体和下丘脑腹内侧。这些区域涉及与社会认知相关的各种功能，包括调节视觉注意力、处理听觉和多模式感觉刺激以及控制对视觉刺激的定向反应。这些结果为了解催产素调节该物种的社会认知和行为的神经机制提供了见解，该物种与人类一样，使用视觉和听觉作为社交交流的主要方式。
The rhesus macaque (Macaca mulatta) is an important primate model for social cognition, and recent studies have begun to explore the impact of oxytocin on social cognition and behavior. Macaques have great potential for elucidating the neural mechanisms by which oxytocin modulates social cognition, which has implications for oxytocin-based pharmacotherapies for psychiatric disorders such as autism and schizophrenia. Previous attempts to localize oxytocin receptors (OXTR) in the rhesus macaque brain have failed due to reduced selectivity of radioligands, which in primates bind to both OXTR and the structurally similar vasopressin 1a receptor (AVPR1A). We have developed a pharmacologically-informed competitive binding autoradiography protocol that selectively reveals OXTR and AVPR1A binding sites in primate brain sections. Using this protocol, we describe the neuroanatomical distribution of OXTR in the macaque. Finally, we use in situ hybridization to localize OXTR mRNA. Our results demonstrate that OXTR expression in the macaque brain is much more restricted than AVPR1A. OXTR is largely limited to the nucleus basalis of Meynert, pedunculopontine tegmental nucleus, the superficial gray layer of the superior colliculus, the trapezoid body, and the ventromedial hypothalamus. These regions are involved in a variety of functions relevant to social cognition, including modulating visual attention, processing auditory and multimodal sensory stimuli, and controlling orienting responses to visual stimuli. These results provide insights into the neural mechanisms by which oxytocin modulates social cognition and behavior in this species, which, like humans, uses vision and audition as the primary modalities for social communication.
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DOI：
10.1126/science.1226201
发表时间：
2012-10-26
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Garrison JL;Macosko EZ;Bernstein S;Pokala N;Albrecht DR;Bargmann CI
通讯作者：
Bargmann CI
影响因子：
5.3
作者：
Bale, TL;Davis, AM;McCarthy, MM
通讯作者：
McCarthy, MM
影响因子：
2.5
作者：
Beery, Annaliese K.;Lacey, Eileen A.;Francis, Darlene D.
通讯作者：
Francis, Darlene D.
影响因子：
1.7
作者：
Kobayashi, Y;Saito, Y;Isa, T
通讯作者：
Isa, T
影响因子：
2.4
作者：
Hawley, ML;Litovsky, RY;Culling, JF
通讯作者：
Culling, JF