Microfluidic Channels on Nanopatterned Substrates: Monitoring Protein Binding to Lipid Bilayers with Surface-Enhanced Raman Spectroscopy.
Microfluidic Channels on Nanopatterned Substrates: Monitoring Protein Binding to Lipid Bilayers with Surface-Enhanced Raman Spectroscopy.
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DOI：
10.1016/j.cplett.2010.02.053
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发表时间：
2010-04-01
影响因子：
2.8
通讯作者：
Grebel, H.
中科院分区：
文献类型：
--
作者：
Banerjee, Amrita;Perez-Castillejos, R.;Hahn, D.;Smirnov, Alex I.;Grebel, H.
我们使用表面增强拉曼光谱（SERS）来检测链霉亲和素和生物素化脂质双层之间的结合事件。结合事件发生在微流控通道和阳极氧化铝（AAO）之间的表面，后者作为基底。脂质双层被纳入基底孔隙中。结果表明，未结合的分子很容易被冲走，而且大的悬浮细胞（肠炎沙门氏菌）不太可能干扰监测过程：当聚焦在通道的下表面时，人们主要可以分辨出结合的分子。
We used Surface Enhanced Raman Spectroscopy (SERS) to detect binding events between streptavidin and biotinylated lipid bilayers. The binding events took place at the surface between microfluidic channels and anodized aluminum oxide (AAO) with the latter serving as substrates. The bilayers were incorporated in the substrate pores. It was revealed that non-bound molecules were easily washed away and that large suspended cells (Salmonella enterica) are less likely to interfere with the monitoring process: when focusing to the lower surface of the channel, one may resolve mostly the bound molecules.
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影响因子：
3.8
作者：
Li, R.;Banerjee, A.;Grebel, H.
通讯作者：
Grebel, H.
影响因子：
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通讯作者：
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通讯作者：
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