Genetic characterization of the chromosome single-segment substitution lines of O. glumaepatula and O. barthii and identification of QTLs for yield-related traits
Genetic characterization of the chromosome single-segment substitution lines of O. glumaepatula and O. barthii and identification of QTLs for yield-related traits
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O. glumaepatula 和 O. barthii 染色体单片段替换系的遗传特征及产量相关性状 QTL 的鉴定
DOI：
10.1007/s11032-019-0960-0
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发表时间：
2019-04
影响因子：
3.1
通讯作者：
Xuelin Fu
中科院分区：
文献类型：
--
作者：
Hanwei Zhao;Lingling Sun;Tianyi Xiong;Zhangqiang Wang;Yu Liao;Tuo Zou;Mingmin Zheng;Zhe Zhang;Xiaoping Pan;Ning He;Guiquan Zhang;Haitao Zhu;Ziqiang Liu;Ping He;Xuelin Fu
颖壳稻和巴氏稻是稻属AA基因组野生种。为了通过远缘杂交有效地从这些野生种中挖掘和转移有益基因，本研究利用O. glumaepatula和O. barthii为供体，籼稻品种HJX 74为受体。共获得168个O. glumaepatula（GLU-SSSL）和O. barthii（BAR-SSSL）的开发。在168个GLU-SSSL和95个BAR-SSSL中，替换片段的总长度分别为3636.35cM和1731.65cM，分布在12条染色体上。因此，每个取代片段的平均长度在GLU-SSSL中为21.64 cM，在BAR-SSSL中为18.23 cM。GLU-SSSLs和BAR-SSSLs的替换片段在水稻基因组上的总覆盖率分别为80.15%和54.67%。为了评估这些SSSL的利用潜力，四个产量相关性状，抽穗期，株高，结实率，和千粒重，在两个赛季的SSSL调查。单穗型自交系间各性状变异显著。在P ≤ 0.05水平下，共检测到28个稳定的GLU-SSSL QTL和53个稳定的BAR-SSSL QTL。其中，12个QTL的加性效应对表型变异的贡献率平均在10%以上，qPHg1-2、qGWTg9-2、qHDb1-1、qPHb 1 -2、qPHb 1 -3、qPHb 7 -1、qPHb 7 -2、qPHb 8 -1、qPHb 10 -1、qPHb 1 -2 -2、qSSb7-1和qSSb10-2。将两个供体的9对QTL定位在第1、5和10染色体上，表明O. glumaepatula和O.巴氏杆菌已被发现并转移到SSSL。此外，qGWTg 1 -1、qGWTg 4 -1和qGWTb 1 -1均表现出正的加性效应，表明来自这3个QTL的2个野生供体的等位基因具有增加SSSL千粒重的潜力。水稻野生种SSSL文库是水稻种质创新和育种的宝贵资源。
Oryza glumaepatula and Oryza barthii are AA-genome wild species in Oryza genus. In order to effectively mine and transfer beneficial genes from these wild species in genome-wide by distant hybridization, in the present study, the chromosome single-segment substitution lines (SSSLs) were developed using O. glumaepatula and O. barthii as donors and elite indica cultivar, HJX74, as a recipient. A total of 168 homozygous SSSLs of O. glumaepatula (GLU-SSSLs) and 95 homozygous SSSLs of O. barthii (BAR-SSSLs) were developed. In 168 GLU-SSSLs and 95 BAR-SSSLs, the total lengths of the substituted segments distributing on 12 chromosomes were 3636.35 cM and 1731.65 cM, respectively. Accordingly, the mean length of each substituted segment was 21.64 cM in GLU-SSSLs and 18.23 cM in BAR-SSSLs. The total coverage lengths of the substituted segments on rice genome were 80.15% in GLU-SSSLs and 54.67% in BAR-SSSLs. To evaluate the utilization potential of these SSSLs, four yield-related traits, heading date, plant height, seed-setting rate, and 1000-grain weight, were investigated in the SSSLs over two seasons. Significant variations of the traits were observed among the SSSLs. At P ≤ 0.05 level, a sum of 28 stable QTLs in GLU-SSSLs and 53 in BAR-SSSLs were identified. Among them, the additive effects of 12 QTLs contributed averagely more than 10% for phenotypic variations, viz., qPHg1-2, qGWTg9-2, qHDb1-1, qPHb1-2, qPHb1-3, qPHb7-1, qPHb7-2, qPHb8-1, qPHb10-1, qPHb12-2, qSSb7-1, and qSSb10-2. Nine pairs of QTLs in the two donors were comparatively mapped on chromosomes 1, 5, and 10, which means the same QTLs or alleles existing in O. glumaepatula and O. barthii have been detected and transferred to SSSLs. Besides, qGWTg1-1, qGWTg4-1, and qGWTb1-1 have shown positive additive effects, implying alleles from the two wild donors of these QTLs have the potential in increasing 1000-grain weight in SSSLs. The SSSLs library of the wild species is a valuable resource for rice germplasm innovation and breeding.
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影响因子：
4.5
作者：
Gao H;Zheng XM;Fei G;Chen J;Jin M;Ren Y;Wu W;Zhou K;Sheng P;Zhou F;Jiang L;Wang J;Zhang X;Guo X;Wang JL;Cheng Z;Wu C;Wang H;Wan JM
通讯作者：
Wan JM
影响因子：
64.8
作者：
Matsumoto, T;Wu, JZ;Sugiura, M
通讯作者：
Sugiura, M
影响因子：
56.9
作者：
Tanksley, SD;McCouch, SR
通讯作者：
McCouch, SR
影响因子：
2.3
作者：
Bhatia, Dharminder;Joshi, Shailesh;Singh, Kuldeep
通讯作者：
Singh, Kuldeep
影响因子：
3.3
作者：
Jinhua Xiao;Jiming Li;S. Grandillo;S. Ahn;L. Yuan;S. Tanksley;S. McCouch
通讯作者：
Jinhua Xiao;Jiming Li;S. Grandillo;S. Ahn;L. Yuan;S. Tanksley;S. McCouch