Ethanol Extracted from Radix of Actinidia Chinensis Inhibits Human Colon Tumor Through Inhibiting Notch-signaling Pathway.
Ethanol Extracted from Radix of Actinidia Chinensis Inhibits Human Colon Tumor Through Inhibiting Notch-signaling Pathway.
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DOI：
10.7150/jca.51275
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发表时间：
2021
影响因子：
3.9
通讯作者：
Zheng C
中科院分区：
文献类型：
--
作者：
Hu W;Wu C;Yuan C;Chen M;Jin C;Zheng C
背景资料：结直肠癌（Colorectal cancer，CRC）是最常见的肿瘤之一，尽管治疗手段不断进步，但其5年生存率仍然很低。猕猴桃根乙醇提取物（EERAC）对人结肠癌细胞有一定的抗肿瘤作用，但其作用机制尚不清楚。方法：采用CCK-8细胞计数试剂盒、创伤愈合和transwell实验检测细胞增殖、迁移和侵袭能力。流式细胞仪检测细胞凋亡和细胞周期。Western印迹和qRT-PCR用于测量靶分子的表达。异种移植瘤实验检测EERAC对肿瘤生长的影响。结果如下：结果表明，EERAC能抑制SW 480细胞的存活、迁移和侵袭能力，并呈浓度依赖性，但能显著促进细胞凋亡和S期细胞比例。使用蛋白质印迹和免疫组织化学染色证实了EERAC对Notch信号通路分子Notch 1、Jagged 1和c-Myc的抑制。EERAC对肿瘤生长有明显的抑制作用。同时，EERAC可显著逆转MAML 1对SW 480细胞存活的影响。结论：EERAC是一种有前途的治疗结直肠癌的化疗药物。
Background: Colorectal cancer (CRC) is one of the most common tumors, and its five-year survival is still very low despite of the advance of treatment strategies. The antitumor effect of ethanol extracted from radix of Actinidia chinensis (EERAC) were identified in human colon cancer cells, but the underlying mechanism remains unclear. Methods: Cell proliferation, migration, and invasion were measured with cell counting kit-8 (CCK-8), wound healing, and transwell assays. Cell apoptosis and cycle were detected by flow cytometry. Western blotting and qRT-PCR were used to measure expression of target molecules. Xenograft tumor assay was applied to detect the influence of EERAC on tumor growth. Results: we found that EERAC inhibited the cell viability, migration, and invasion of SW480 cells in a concentration dependent manner, but promoted apoptosis and the cell percentage in S phase significantly. The suppression of notch-signaling pathway molecules, Notch1, Jagged1, and c-Myc, by EERAC was confirmed using western blotting and immunohistochemical staining. The significant inhibition of tumor growth by EERAC was also observed. Meanwhile, EERAC remarkably reversed the effects of mastermind like transcriptional coactivator 1 (MAML1, activator of notch-signaling pathway) on cell survival of SW480. Conclusions: EERAC might be a promising chemotherapeutic agent for CRC treatment.
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DOI：
10.1038/nrg3272
发表时间：
2012-09
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
--
影响因子：
--
作者：
Li, Bo;Zhang, Wei;Ge, Zhengxing
通讯作者：
Ge, Zhengxing
影响因子：
6.1
作者：
Lv, Jiapei;Wang, Lingyan;Wang, Xiangdong
通讯作者：
Wang, Xiangdong
DOI：
10.19540/j.cnki.cjcmm.20171012.004
发表时间：
2017-12-01
期刊：
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
影响因子：
--
作者：
Ma, Kun;Li, Min
通讯作者：
Li, Min
影响因子：
20.3
作者：
Jundt, F;Anagnostopoulos, I;Dörken, B
通讯作者：
Dörken, B